华西口腔医学杂志 ›› 2012, Vol. 30 ›› Issue (5): 514-517.doi: 10.3969/j.issn.1000-1182.2012.05.015

• 临床研究 • 上一篇    下一篇

245例人类免疫缺陷病毒感染者唾液中人类疱疹病毒1~4型的检测情况分析

吴凡1 翟维维1 葛柳莹1 祁燕伟2 高辉3 段开文1   

  1. 1.昆明医科大学附属延安医院昆明市延安医院口腔科, 昆明650051;2.昆明市第三人民医院艾滋病科, 昆明650041;3.昆明医科大学附属延安医院昆明市延安医院检验科, 昆明650051
  • 收稿日期:2011-08-16 修回日期:2012-07-05 出版日期:2012-10-01 发布日期:2012-10-01
  • 通讯作者: 段开文,Tel:0871-3211142
  • 作者简介:吴凡(1985—),女,湖北人,硕士
  • 基金资助:

    国家自然科学基金资助项目(30860315);云南省应用基础研究基金资助项目(2008ZC146M)

Incidence of human herpes virus 1-4 type in saliva of 245 human immunodeficiency virus-infected patients

Wu Fan1, Zhai Weiwei1, Ge Liuying1, Qi Yanwei2, Gao Hui3, Duan Kaiwen1.   

  1. 1. Dept. of Stomatology, Kunming Medical University Yan’an Hospital, Kunming Yan’an Hospital, Kunming 650051, China; 2. Dept. of AIDS, Kunming Third Hospital, Kunming 650041, China; 3. Dept. of Laboratory Medicine, Kunming Medical University Yan’an Hospital, Kunming Yan’an Hospital, Kunming 650051, China
  • Received:2011-08-16 Revised:2012-07-05 Online:2012-10-01 Published:2012-10-01

摘要:

目的探讨人类免疫缺陷病毒(HIV)感染者唾液中人类疱疹病毒(HHV)1~4型即单纯疱疹病毒1型(HSV-1)、单纯疱疹病毒2型(HSV-2)、水痘-带状疱疹病毒(VZV)和EB病毒(EBV)的分布情况。方法利用聚合酶链反应(PCR)方法对HIV感染者和健康者非刺激性全唾液中HSV-1、HSV-2、VZV和EBV DNA的存在情况进行检测,并采用SPSS 18.0软件建立数据库并进行相关指标的统计分析。结果在245例HIV阳性患者唾液中,HSV-1、HSV-2、VZV和EBV检出率分别为29.0%、3.3%、4.1%和82.0%;30例对照组唾液中上述4种病毒检出率分别为13.3%、0、0和36.7%。两组总体检出率比较差异有统计学意义(P<0.01)。高效抗逆转录病毒治疗(HAART)使用后HIV感染者唾液中4种病毒的检出率与未使用HAART的患者差异均无统计学意义(P>0.05)。结论云南地区HIV感染者HHV有较高的流行率,以EBV最为常见,其次为HSV-1,VZV和HSV-2少见,还存在多种疱疹病毒联合感染。

关键词: 人类免疫缺陷病毒, 唾液, 高效抗逆转录病毒治疗, 人类疱疹病毒

Abstract:

Objective To determine the incidence of human herpes virus(HHV) 1-4 type including herpes simplex virus type-1(HSV-1), herpes simplex virus type-2(HSV-2), varicella-zoster virus(VZV), Epstein-Barr virus(EBV)in the saliva of human immunodeficiency virus(HIV)-infected patients. Methods The incidence of salivary HSV-1, HSV-2, VZV and EBV from 245 HIV-seropositive individuals and control group was used to investigate by polymerase chain reaction(PCR) or nested PCR. The data was analyzed by SPSS 18.0 statistical software. Results In the 245 HIV-seropositive individuals, the detection rates of HSV-1, HSV-2, VZV, EBV were 29.0%, 3.3%, 4.1%, 82.0%. In the control group, the detection rates of HSV-1, HSV-2, VZV, EBV were 13.3%, 0, 0, 36.7%. Four HHVs were significantly
more prevalent in the salivas of HIV-seropositive persons than those in the control group(P<0.01). The detection rates of HSV-1, HSV-2, VZV and EBV DNA were no difference between the HIV-positive group with highly active antiretroviral therapy(HAART) and HIV-positive group without HAART(P>0.05). Conclusion There is a high prevalence of HHV infection in HIV-infected people in Yunnan. The most common virus are EBV, followed by HSV-1, but VZV and HSV-2 are rarely detected. HHV co-infection is also observed.

Key words: human immunodeficiency virus, saliva, highly active antiretroviral therapy, human herpes virus