微纳米共存的磷酸化涂层对钛植入体骨结合的影响

doi:10.7518/hxkq.2021.05.006

华西口腔医学杂志 ›› 2021, Vol. 39 ›› Issue (5): 531-539.doi: 10.7518/hxkq.2021.05.006

微纳米共存的磷酸化涂层对钛植入体骨结合的影响

张洁(), 祝颂松, 姜楠()

口腔疾病研究国家重点实验室国家口腔疾病临床医学研究中心四川大学华西口腔医院正颌及关节外科，成都 610041

收稿日期:2020-12-21 修回日期:2021-04-19 出版日期:2021-10-01 发布日期:2021-10-11
通讯作者: 姜楠 E-mail:ttxsjiezhang@163.com;dent_jn@163.com
作者简介:张洁，硕士，E-mail：ttxsjiezhang@163.com
基金资助:
国家自然科学基金青年基金(81901026)

Effect of micro/nanoscaled Ti phosphate/Ti oxide hybrid coating on the osseointegration of Ti implants

Zhang Jie(), Zhu Songsong, Jiang Nan.()

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Orthognathic and Temporomandibular Joint Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China

Received:2020-12-21 Revised:2021-04-19 Online:2021-10-01 Published:2021-10-11
Contact: Jiang Nan. E-mail:ttxsjiezhang@163.com;dent_jn@163.com
Supported by:
The National Natural Science Foundation of China(81901026);Correspondence: Jiang Nan, E-mail: dent_jn@163.com

摘要/Abstract

摘要： 目的

在纯钛植入体表面制备出微纳米共存的具备晶体相“磷”的仿生结构，探究其对钛植入体骨结合的影响。

方法

利用特殊压强下碱热磷酸反应法在纯钛表面制备出微纳米共存的磷酸化涂层，即钛磷钛（TiP-Ti），选取未处理的光滑纯钛（cp-Ti）作为对照。对材料进行表征分析，并利用体外细胞学技术探究TiP-Ti对大鼠骨髓间充质干细胞（BMSCs）增殖、黏附以及分化的影响。最后，将材料植入大鼠体内，12周后评估TiP-Ti对宿主骨结合的影响。

结果

TiP-Ti表面呈现纳米级的3D空间结构与微米级孔隙共存的仿生结构。体外实验显示BMSCs在TiP-Ti表面具有更好的黏附、增殖和成骨向分化。体内实验显示，TiP-Ti诱导了与宿主组织的更牢固的界面结合，极限抗剪切强度和最大推出力显著提高。

结论

本研究成功构建了TiP-Ti微纳米共存的仿生结构，促进了植入体的骨结合能力，是一种具有应用前景的植入体表面改性。

关键词: 磷酸钛, 氧化钛, 骨结合, 微纳米表面形态, 分层结构

Abstract: Objective

This study was performed to fabricate a bionic coating with titanium (Ti) phosphate to promote the osseointegration of Ti substrate implants.

Methods

Phosphorylated micro/nanocoating was prepared on the surface of pure titanium (i.e., TiP-Ti) by hydrothermal process under special pressure, and the untreated smooth pure titanium (cp-Ti) was selected as the control. To evaluate the characteristics of the coating surface, scanning electron microscopy, X-ray diffraction, atomic force microscopy, and contact-angle measurement were performed. In addition, the effects of TiP-Ti on the proliferation, adhesion, and differentiation of rat bone marrow mesenchymal stem cells (BMSCs) were investigated by using in vitro cytology. Finally, TiP-Ti implants were implanted into the rat tibia, and the effect of TiP-Ti on the osseointegration in the host was evaluated after 12 weeks.

Results

The TiP-Ti surface presented a bionic structure with coexisting nanoscale 3D spatial structure and microscale pores. In vitro experiments showed that the BMSCs had enhanced adhesion, proliferation, and osteogenic differentiation on the TiP-Ti surface. Furthermore, in vivo, TiP-Ti showed considerably stronger osseointegration compared with pure titanium, and the ultimate shear strength and maximum pushing force were significantly improved.

Conclusion

A bionic structure with TiP-Ti micro/nanoscale coating was successfully fabricated, indicating a promising method for modifying the surface of implants.

Key words: Ti phosphate, Ti oxide, osseointegration, micro/nanoscale morphology, hierarchical structure

中图分类号:

R 783.4

张洁, 祝颂松, 姜楠. 微纳米共存的磷酸化涂层对钛植入体骨结合的影响[J]. 华西口腔医学杂志, 2021, 39(5): 531-539.

Zhang Jie, Zhu Songsong, Jiang Nan.. Effect of micro/nanoscaled Ti phosphate/Ti oxide hybrid coating on the osseointegration of Ti implants[J]. West China Journal of Stomatology, 2021, 39(5): 531-539.

图/表 13

图 1

图 2

表 1

图 3

图 4

图 5

图 6

图 7

图 8

图 9

图 10

图 11

表 2

参考文献 25

1	武钺, 金建烽. 加载氧化石墨烯纯钛的表面性状及其对细菌黏附和成骨细胞结构的影响[J]. 华西口腔医学杂志, 2019, 37(4): 366-371.
	Wu Y, Jin JF. Surface characteristics of pure titanium loaded graphene oxide: effect on bacteria adhesion and osteoblast structure[J]. West China J Stomatol, 2019, 37(4): 366-371.
2	Lee BH, Lee C, Kim DG, et al. Effect of surface structure on biomechanical properties and osseointegration[J]. Mater Sci Engg C Biom Supramol System, 2008, 28(8): 1448-1461.
3	Le Guéhennec L, Soueidan A, Layrolle P, et al. Surface treatments of titanium dental implants for rapid osseointegration[J]. Dent Mater, 2007, 23(7): 844-854.
4	Junker R, Dimakis A, Thoneick M, et al. Effects of implant surface coatings and composition on bone integration: a systematic review[J]. Clin Oral Implants Res, 2009, 20(): 185-206.
5	Geurs NC, Jeffcoat RL, McGlumphy EA, et al. Influen-ce of implant geometry and surface characteristics on progressive osseointegration[J]. Int J Oral Maxillofac Implants, 2002, 17(6): 811-815.
6	Bjursten LM, Rasmusson L, Oh S, et al. Titanium dioxide nanotubes enhance bone bonding in vivo[J]. J Bio-med Mater Res A, 2010, 92(3): 1218-1224.
7	Sul YT. The significance of the surface properties of oxidized titanium to the bone response: special emphasis on potential biochemical bonding of oxidized titanium implant[J]. Biomaterials, 2003, 24(22): 3893-3907.
8	Park JW, Kim YJ, Jang JH, et al. Surface characteristics and primary bone marrow stromal cell response of a na-nostructured strontium-containing oxide layer produced on a microrough titanium surface[J]. J Biomed Mater Res A, 2012, 100(6): 1477-1487.
9	Park JW, Jang JH, Lee CS, et al. Osteoconductivity of hydrophilic microstructured titanium implants with phosphate Ion chemistry[J]. Acta Biomater, 2009, 5(6): 2311-2321.
10	Humphrey GL. The heats of formation of TiO, Ti₂O₃, Ti₃O₅ and TiO₂ from combustion calorimetry[J]. J Am Chem Soc, 1951, 73(4): 1587-1590.
11	Yada M, Inoue Y, Sakamoto A, et al. Synthesis and controllable wettability of Micro- and nanostructured tita-nium phosphate thin films formed on titanium plates[J]. ACS Appl Mater Interfaces, 2014, 6(10): 7695-7704.
12	李莺, 尤亚鹏, 李宝娥, 等. 纯钛表面TiO₂纳米管结合Ⅰ型胶原促进成骨细胞黏附和骨结合[J]. 中国组织工程研究, 2019, 23(14): 2169-2176.
	Li Y, You YP, Li BE, et al. Type Ⅰ collagen combined titanium dioxide nanotube composite coating modified titanium surface improves osteoblast adhesion and osseointegration[J]. J Clin Rehabil Tis Eng Res, 2019, 23(14): 2169-2176.
13	Neel EA, Chrzanowski W, Salih VM, et al. Tissue engineering in dentistry[J]. J Dent, 2014, 42(8): 915-928.
14	Meng WX, Takeichi M. Adherens junction: molecular architecture and regulation[J]. Cold Spring Harb Perspect Biol, 2009, 1(6): a002899.
15	Ishizaki T, Saito N, Takai O. Correlation of cell adhesive behaviors on superhydrophobic, superhydrophilic, and micropatterned superhydrophobic/superhydrophilic surfaces to their surface chemistry[J]. Langmuir, 2010, 26(11): 8147-8154.
16	董菲, 丁仲鹃, 牛涛. TiO₂喷砂酸蚀处理对钛片表面氧化膜及成骨细胞生长影响的研究[J]. 华西口腔医学杂志, 2008, 26(1): 10-14.
	Dong F, Ding ZJ, Niu T. Effects of TiO₂ blasted and acid-etched titanium surfaces on oxide-film and osteoblast[J]. West China J Stomatol, 2008, 26(1): 10-14.
17	Hu DD, Li K, Xie YT, et al. The combined effects of na-notopography and Sr ion for enhanced osteogenic activity of bone marrow mesenchymal stem cells (BMSCs)[J]. J Biomater Appl, 2017, 31(8): 1135-1147.
18	Jiang N, Du PG, Qu WD, et al. The synergistic effect of TiO₂ nanoporous modification and platelet-rich plasma treatment on titanium-implant stability in ovariectomized rats[J]. Int J Nanomedicine, 2016, 11: 4719-4733.
19	Chesnutt BM, Viano AM, Yuan YL, et al. Design and characterization of a novel chitosan/nanocrystalline calcium phosphate composite scaffold for bone regeneration[J]. J Biomed Mater Res A, 2009, 88(2): 491-502.
20	Gildenhaar R, Knabe C, Gomes C, et al. Calcium alkaline phosphate scaffolds for bone regeneration 3D-rabricated by additive manufacturing[J]. Key Eng Mater, 2012, 493/494: 849-854.
21	Nagasawa M, Cooper LF, Ogino Y, et al. Topography influences adherent cell regulation of osteoclastogenesis[J]. J Dent Res, 2016, 95(3): 319-326.
22	Zhou YL, Marson RL, van Anders G, et al. Biomimetic hierarchical assembly of helical supraparticles from chiral nanoparticles[J]. ACS Nano, 2016, 10(3): 3248-3256.
23	Filova E, Suchy T, Sucharda Z, et al. Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and mi-cro-size hydroxyapatite in composites[J]. Int J Nanomed, 2014, 2014: 3687-3706.
24	Woo KM, Jun JH, Chen VJ, et al. Nano-fibrous scaffol-ding promotes osteoblast differentiation and biominera-lization[J]. Biomaterials, 2007, 28(2): 335-343.
25	Li YF, Zou SJ, Wang DZ, et al. The effect of hydrofluo-ric acid treatment on titanium implant osseointegration in ovariectomized rats[J]. Biomaterials, 2010, 31(12): 3266-3273.

项目	cp-Ti	TiP-Ti	t值	P值
RMS/nm	6.35±2.4	58.65±8.2	3.640	0.010
Z范围/nm	182.83±22.45	569.04±50.43	2.450	0.020
表面积差/%	4.23±0.88	32.54±4.56	3.640	0.010

项目	cp-Ti	TiP-Ti	t值	P值
最大推出力/N	34.5±3.5	182.7±10.2	3.250	0.010
极限抗剪切强度/（N·mm^-2）	9.63±0.25	22.87±0.55	4.297	0.020

[1]	敖小刚, 陈文川. 低温等离子体促进钛种植体骨结合的研究进展[J]. 华西口腔医学杂志, 2020, 38(5): 566-570.
[2]	王亚楠, 贾婷婷, 徐欣, 张东姣. 系统性药物对种植体骨结合影响的动物实验研究进展[J]. 华西口腔医学杂志, 2020, 38(2): 211-217.
[3]	罗翠芬, 彭国光. 生骨片对后牙区种植体骨结合的影响[J]. 华西口腔医学杂志, 2017, 35(5): 498-501.
[4]	王买全彭利伟李运峰. 全身应用催产素对骨质疏松大鼠种植体骨结合的影响[J]. 华西口腔医学杂志, 2016, 34(4): 332-335.
[5]	李欣姜志红柳忠豪. 浓缩生长因子提取液对钛片表面MC3T3-E1细胞增殖分化的影响[J]. 华西口腔医学杂志, 2015, 33(1): 84-87.
[6]	曾永发石连水戴群张林孔滢. 添加载银纳米二氧化钛光亮漆体外细胞毒性及其抗菌性的实验研究[J]. 华西口腔医学杂志, 2014, 32(6): 611-615.
[7]	刘杰葛亚丽徐连立. 载银纳米二氧化钛树脂基托对变异链球菌和白色假丝酵母菌抗菌性的体外研究[J]. 华西口腔医学杂志, 2012, 30(2): 201-205.
[8]	周屹立丁仲鹃唐玲. 钛片表面粗糙度和氧化膜对成骨细胞黏附影响的体外研究[J]. 华西口腔医学杂志, 2011, 29(01): 13-16.
[9]	李剑平张望群于静戚孟春胡静王东胜. 实验性骨质疏松对种植体周自体骨移植愈合影响的研究[J]. 华西口腔医学杂志, 2010, 28(04): 435-438.
[10]	尹路惠迎雪姚江武. 氮硅锆-羟磷灰石涂层应用于纯钛种植体的实验研究[J]. 华西口腔医学杂志, 2009, 27(05): 492-495.

微纳米共存的磷酸化涂层对钛植入体骨结合的影响

Effect of micro/nanoscaled Ti phosphate/Ti oxide hybrid coating on the osseointegration of Ti implants

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 13

参考文献 25

相关文章 10

编辑推荐

Metrics

本文评价