华西口腔医学杂志 ›› 2021, Vol. 39 ›› Issue (4): 434-440.doi: 10.7518/hxkq.2021.04.009

• 基础研究 • 上一篇    下一篇

Ki-67Cyclin D1P53P16在成都地区有辛辣饮食习惯的白斑及癌变患者中的表达

万梓欣(), 郑志建, 黄美畅, 陈宇, 姚莉洪()   

  1. 口腔疾病研究国家重点实验室 国家口腔疾病临床医学研究中心 四川大学华西口腔医院病理科,成都 610041
  • 收稿日期:2020-05-06 修回日期:2021-03-16 出版日期:2021-08-01 发布日期:2021-08-10
  • 通讯作者: 姚莉洪 E-mail:wanzixinhxkq@126.com;yaolihongsansan@126.com
  • 作者简介:万梓欣,技师,学士,E-mail:wanzixinhxkq@126.com
  • 基金资助:
    四川大学华西口腔医院临床研究项目(LCYJ2019-8)

Expression of Ki-67, Cyclin D1, P53, and P16 in patients with oral leukoplakia and leukoplakia cancerization with spicy diet in Chengdu

Wan Zixin(), Zheng Zhijian, Huang Meichang, Chen Yu, Yao Lihong()   

  1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Oral Pathology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2020-05-06 Revised:2021-03-16 Online:2021-08-01 Published:2021-08-10
  • Contact: Yao Lihong E-mail:wanzixinhxkq@126.com;yaolihongsansan@126.com
  • Supported by:
    Clinical Research Project of West China Hospital of Stomatology of Sichuan University(LCYJ2019-8)

摘要: 目的

探讨Ki-67、Cyclin D1、P53和P16在成都地区有辛辣饮食习惯的白斑(OLK)及OLK癌变患者中的表达。

方法

将成都地区有辛辣饮食习惯的30例OLK患者和无辛辣饮食习惯的15例OLK患者,分别分成OLK不伴上皮异常增生(OLK-)、OLK伴轻-中度上皮异常增生(OLK+)、OLK伴重度上皮异常增生或癌变(OLK++/OSCC)3组,采用免疫组化检测Ki-67、Cyclin D1、P53和P16在各组的表达,并对结果进行统计分析。

结果

在有/无辛辣饮食习惯的OLK及癌变患者中,Ki-67和P53在OLK+组和OLK++/OSCC组的表达高于OLK-组(P<0.05),Cyclin D1在OLK++/OSCC组的表达高于OLK-组(P<0.05),P16在OLK++/OSCC组的表达低于OLK-组(P<0.05)。Ki-67、Cyclin D1、P53、P16在有和无辛辣饮食习惯的OLK患者中的表达差异无统计学意义(P>0.05)。Ki-67与Cyclin D1的表达成正相关(r=0.439,P=0.015)。

结论

辛辣饮食习惯对OLK及癌变患者Ki-67、Cyclin D1、P53和P16的表达无影响,但是Ki-67、Cyclin D1和P53表达随OLK病变的进展而升高,P16表达随病变进展而降低。

关键词: 辛辣饮食, 口腔白斑, 癌变, 口腔黏膜潜在恶性疾患

Abstract: Objective

To investigate the expression of Ki-67, Cyclin D1, P53, and P16 in patients with oral leukoplakia (OLK) and OLK cancerization who have aspicy diet in Chengdu.

Methods

Thirtypatients with OLK andspicy diet and 15 patients with OLK without spicy diet in Chengdu were divided into three groups: hyperplastic OLK (OLK-), OLK with mild to moderate dysplasia (OLK+), and severe dysplastic  OLK or oral squamous cell carcinoma (OSCC) transforming from OLK (OLK++/OSCC). The expression of Ki-67, Cyclin D1, P53, and P16 were detected by immunohistochemistry and statistically analyzed.

Results

The expression of Ki-67 and P53 in patients with or without spicy diet in the OLK+and OLK++/OSCC groups were stronger than that of the OLK- group (P<0.05). The OLK++/OSCC group showed a higher expression of Cyclin D1 and lower expression of P16 than the OLK- group (P<0.05). The expression of Ki-67, Cyclin D1, P53, and P16 in patients with spicy diet and without spicy diet had no substantial difference. The expression of Ki-67 and Cyclin D1 showed a positive correlation (r=0.439, P=0.015).

Conclusion

Spicy diet did not have an influence on the expression of Ki-67, Cyclin D1, P53, and P16 in patients with OLK and OSCC. The expression of Ki-67, Cyclin D1, and P53 increased with the development of OLK, whereas P16 showed opposite expression trend.

Key words: spicy diet, oral leukoplakia, cancerization, oral potentially malignant disorders

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