华西口腔医学杂志 ›› 2021, Vol. 39 ›› Issue (4): 425-433.doi: 10.7518/hxkq.2021.04.008

• 基础研究 • 上一篇    下一篇

circ_0005379通过调控miR-17-5p/酰基辅酶A氧化酶1轴抑制口腔鳞状细胞癌的发展进程

周海霞(), 王璐瑶, 陈帅, 王丹丹, 方政()   

  1. 郑州大学第一附属医院口腔医学中心,郑州 450052
  • 收稿日期:2020-06-26 修回日期:2021-04-12 出版日期:2021-08-01 发布日期:2021-08-10
  • 通讯作者: 方政 E-mail:zdyfyzhhx0408@163.com;ajsgamryttlj@163.com
  • 作者简介:周海霞,主治医师,硕士,E-mail:zdyfyzhhx0408@163.com

circ_0005379 inhibits the progression of oral squamous cell carcinoma by regulating the miR-17-5p/acyl-CoA oxidase 1 axis

Zhou Haixia(), Wang Luyao, Chen Shuai, Wang Dandan, Fang Zheng()   

  1. Center of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
  • Received:2020-06-26 Revised:2021-04-12 Online:2021-08-01 Published:2021-08-10
  • Contact: Fang Zheng E-mail:zdyfyzhhx0408@163.com;ajsgamryttlj@163.com

摘要: 目的

探讨circ_0005379对口腔鳞状细胞癌(OSCC)细胞增殖、凋亡、迁移和侵袭的影响及作用机制。

方法

分别以癌旁正常组织及正常口腔黏膜细胞HOK-16A为对照,实时定量聚合酶链反应(RT-qPCR)检测OSCC组织和SCC15细胞中circ_0005379和miR-17-5p表达水平,蛋白印迹(Western blot)检测酰基辅酶A氧化酶1(ACOX1)蛋白表达水平。转染circ_0005379过表达载体至SCC15细胞,四甲基噻唑蓝(MTT)染色法、流式细胞术、Transwell和Western blot分别检测过表达circ_0005379对SCC15细胞增殖、凋亡、迁移和侵袭及上皮性钙黏附蛋白(E-cadherin)、β连环素(β-catenin)和Snail蛋白表达的影响。双荧光素酶报告基因实验检验SCC15细胞中circ_0005379与miR-17-5p及miR-17-5p与ACOX1调控关系。建立稳定过表达circ_0005379的SCC15细胞裸鼠移植瘤模型,观察过表达circ_0005379对裸鼠移植瘤生长的影响。

结果

与癌旁组织比较,OSCC组织中circ_0005379和ACOX1蛋白表达降低(P<0.05),miR-17-5p表达升高(P<0.05)。与HOK-16A细胞比较,SCC15细胞中circ_0005379和ACOX1蛋白表达降低(P<0.05),miR-17-5p表达升高(P<0.05)。过表达circ_0005379后,SCC15细胞活性、迁移和侵袭细胞数及β-catenin和Snail蛋白表达降低(P<0.05),凋亡率和E-cadherin蛋白表达升高(P<0.05)。circ_0005379靶向负调控miR-17-5p表达,ACOX1是miR-17-5p的靶基因。过表达miR-17-5p可逆转过表达circ_0005379对OSCC细胞系增殖、凋亡、迁移和侵袭的影响。过表达circ_0005379的裸鼠肿瘤体积和重量降低(P<0.05),肿瘤组织中circ_0005379和ACOX1蛋白表达水平升高(P<0.05),miR-17-5p表达水平降低(P<0.05)。

结论

circ_0005379可能通过靶向miR-17-5p/ACOX1来抑制体外OSCC细胞增殖、迁移和侵袭,并促进细胞凋亡,同时抑制体内肿瘤生长,其可能是OSCC治疗的潜在靶点。

关键词: 口腔鳞状细胞癌, circ_0005379, miR-17-5p, 酰基辅酶A氧化酶1, 增殖, 凋亡, 迁移, 侵袭

Abstract: Objective

To investigate the effects of circ_0005379 on the proliferation, apoptosis, migration, and invasion of oral squamous cell carcinoma (OSCC) cells and its mechanism.

Methods

Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression levels of circ_0005379 and miR-17-5p in OSCC tissues and SCC15 cell lines. Western blot was used to detect the expression levels of acyl-CoA oxidase 1 (ACOX1). The circ_0005379 overexpression vector was transfected into SCC15 cells. Methyl thiazolyl tetrazolium blue staining, flow cytometry, Transwell, and Western blot were used to detect the effects of circ_0005379 overexpression on the proliferation, apoptosis, migration, and invasion of SCC15 cells and the expression of E-cadherin, β-catenin, and Snail proteins. Dual luciferase reporter assay and RNA immunoprecipitation were used to examine the regulation of circ_0005379, miR-17-5p, miR-17-5p, and ACOX1 in SCC15 cells. A nude mouse xenograft model of SCC15 cells stably overexpressing circ_0005379 was established, and the effect of circ_0005379 overexpression on the growth of xenografts in nude mice was observed.

Results

Compared with adjacent cancer tissues, the expression levels of circ_0005379 and ACOX1 proteins in OSCC tissues were decreased (P<0.05), and the expression level of miR-17-5p was increased (P<0.05). Compared with HOK-16A cells, the expression levels of circ_0005379 and ACOX1 proteins in SCC15 cell lines were decreased (P<0.05), and the expression level of miR-17-5p was increased (P<0.05). After overexpressing circ_0005379, the activity and number of migrating and invading SCC15 cells and the expression levels of β-catenin and Snail proteins were decreased (P<0.05); however, the apoptosis rate and expression level of E-cadherin protein were increased (P<0.05). In SCC15 cells, circ_0005379 targeted the negative regulation of miR-17-5p expression, and miR-17-5p targeted the negative regulation of ACOX1 expression. Overexpressing miR-17-5p or silencing ACOX1 could reverse the effects of circ_0005379 overexpression on the proliferation, apoptosis, migration, and invasion of OSCC cell lines. The tumor volume and weight of nude mice overexpressing circ_0005379 were decreased (P<0.05), the expression levels of circ_0005379 and ACOX1 protein in tumor tissues were increased (P<0.05), and the expression level of miR-17-5p was decreased (P<0.05).

Conclusion

circ_0005379 may inhibit the proliferation, migration, and invasion of OSCC cells by downregulating the expression of miR-17-5p and upregulating ACOX1, which promote apoptosis and inhibit tumor growth in vivo. circ_0005379 may be a potential target for OSCC treatment.

Key words: oral squamous cell carcinoma, circ_0005379, miR-17-5p, acyl-CoA oxidase 1, proliferation, apoptosis, migration, invasion

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