华西口腔医学杂志 ›› 2021, Vol. 39 ›› Issue (4): 405-412.doi: 10.7518/hxkq.2021.04.005

• 基础研究 • 上一篇    下一篇

CXC亚家族趋化因子配体10-受体3/CC亚家族趋化因子配体17-受体4轴在口腔扁平苔藓发病机制中的交互作用

唐楠(), 张雨垚, 程珏华, 赵知白, 范媛()   

  1. 南京医科大学附属口腔医院口腔黏膜病科,江苏省口腔疾病研究重点实验室,江苏省口腔转化医学工程研究中心,南京 210029
  • 收稿日期:2020-07-30 修回日期:2021-01-25 出版日期:2021-08-01 发布日期:2021-08-10
  • 通讯作者: 范媛 E-mail:tang19950819@163.com;fanyuan@ njmu.edu.cn;fanyuan@njmu.edu.cn
  • 作者简介:唐楠,硕士,E-mail:tang19950819@163.com
  • 基金资助:
    国家自然科学基金(81470748);江苏高校优势学科建设工程资助项目(2018-87)

Cross-talk between CXC chemokine ligand 10-CXC chemokine receptor 3 axis and CC chemokine ligand 17-CC chemokine receptor 4 axis in the pathogenesis of oral lichen planus

Tang Nan(), Zhang Yuyao, Cheng Juehua, Zhao Zhibai, Fan Yuan()   

  1. Dept. of Oral Mucosal Diseases, The Affiliated Stomatological Hospital of Nanjing Medical University, Jiangsu Province Key Laboratory of Oral Diseases, Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing 210029, China
  • Received:2020-07-30 Revised:2021-01-25 Online:2021-08-01 Published:2021-08-10
  • Contact: Fan Yuan E-mail:tang19950819@163.com;fanyuan@ njmu.edu.cn;fanyuan@njmu.edu.cn
  • Supported by:
    The National Natural Science Foundation of China(81470748);The Priority Academic Program Development of Jiangsu Higher Education Institutions(2018-87)

摘要: 目的

探讨CXC亚家族趋化因子配体10(CXCL10)-CXC亚家族趋化因子受体3(CXCR3)、CC亚家族趋化因子配体17(CCL17)-CC亚家族趋化因子受体4(CCR4)两轴间在口腔扁平苔藓(OLP)发病机制中的交互关系。

方法

收集OLP患者(非糜烂、糜烂型)和健康对照者外周血,分离T细胞并鉴定纯度,分为空白(不加拮抗剂)、拮抗CXCR3(加入CXCR3拮抗剂)、拮抗CCR4(加入CCR4拮抗剂)三个组与T细胞共培养,流式细胞术检测各组受体CXCR3、CCR4表达,生物素双抗体夹心酶联免疫吸附法检测相应配体CXCL10、CCL17的表达。

结果

T细胞纯度鉴定均>95%且各组间的差异无统计学意义(P>0.05)。受体表达结果显示,OLP中拮抗CXCR3和CCR4组与空白组相比,拮抗CXCR3组的CXCR3和CCR4表达均下调(P>0.05);拮抗CCR4组的CCR4表达显著下调(P<0.05),CXCR3表达上调(P>0.05)。配体表达结果示,OLP中拮抗组与空白组相比,拮抗CXCR3组的CXCL10表达显著下调(P<0.05),CCL17表达也下调(P>0.05);拮抗CCR4组的CCL17表达显著下调(P<0.05),CXCL10表达上调(P>0.05)。健康对照者受体和配体趋势与OLP一致,但拮抗组与空白组相比差异无统计学意义(P>0.05)。

结论

本实验结果提示两轴间在OLP的发病机制中存在相关互作,且可能在OLP的发生发展过程中发挥不同作用。

关键词: 口腔扁平苔藓, CXC亚家族趋化因子受体3, CXC亚家族趋化因子配体10, CC亚家族趋化因子受体4, CC亚家族趋化因子配体17

Abstract: Objective

This study aimed to determine whether a correlation existed between CXC chemokine ligand 10 (CXCL10)-CXC chemokine receptor 3 (CXCR3) and CC chemokine ligand 17 (CCL17)-CC chemokine receptor 4 (CCR4) in the pathogenesis of oral lichen planus (OLP).

Methods

Peripheral blood of OLP patients (non-erosive and erosive groups) and healthy controls were collected, and T cells were isolated and purified. T cells were co-cultured with three groups: blank, anti-CXCR3, and anti-CCR4. CXCR3 and CCR4 expression were detected by flow cytometry, and CXCL10 and CCL17 were detected by enzyme-linked immunosorbent assay, respectively.

Results

The purities of T cells were all >95% in the three groups (P>0.05). Receptor expression showed that CXCR3 and CCR4 in the anti-CXCR3 group was downregulated in OLP compared with the blank group (P>0.05). The level of CCR4 in the anti-CCR4 group was significantly downregulated (P<0.05), and CXCR3 was upregulated (P>0.05). Ligand analysis results showed that CXCL10 in the anti-CXCR3 group was significantly downregulated in OLP compared with the blank group (P<0.05), and CCL17 was also downregulated (P>0.05). CCL17 in the anti-CCR4 group was significantly downregulated (P<0.05), and CXCL10 was upregulated (P>0.05). The trend of receptors and ligands in controls was consistent with OLP, but no significant difference existed between the antagonistic and the blank groups (P>0.05).

Conclusion

Two axes interact with each other in the pathogenesis of OLP and may play different roles in its occurrence and development.

Key words: oral lichen planus, CXC chemokine receptor 3, CXC chemokine ligand 10, CC chemokine receptor 4, CC chemokine ligand 17

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