低龄儿童龋病相关的唾液生化指标研究

doi:10.7518/hxkq.2021.03.009

华西口腔医学杂志 ›› 2021, Vol. 39 ›› Issue (3): 300-305.doi: 10.7518/hxkq.2021.03.009

低龄儿童龋病相关的唾液生化指标研究

张颖¹^,²(), 贾松菠³, 李帆¹^,², 李姗姗¹, 张利娟¹^,², 谭凯璇², 卢洁², 杨芳²()

^1.青岛大学口腔医学院，青岛 266003
^2.青岛市市立医院口腔医学中心，青岛 266071
^3.天津市儿童医院口腔科，天津 300074

收稿日期:2020-05-26 修回日期:2021-02-13 出版日期:2021-06-01 发布日期:2021-05-26
通讯作者: 杨芳 E-mail:15162731585@163.com;yangf82@sina.com
作者简介:张颖，硕士，E-mail：15162731585@163.com
基金资助:
国家自然科学基金面上项目(81670979)

Salivary biochemical indices related to early childhood caries

Zhang Ying¹^,²(), Jia Songbo³, Li Fan¹^,², Li Shanshan¹, Zhang Lijuan¹^,², Tan Kaixuan², Lu Jie², Yang Fang²()

^1.School of Stomatology, Qingdao University, Qingdao 266003, China
^2.Stomatology Center, Qingdao Municipal Hospital, Qingdao 266071, China
^3.Dept. of Stomatology, Tianjin Children, s Hospital, Tianjin 300074, China

Received:2020-05-26 Revised:2021-02-13 Online:2021-06-01 Published:2021-05-26
Contact: Yang Fang E-mail:15162731585@163.com;yangf82@sina.com
Supported by:
The National Natural Science Foundation of China(81670979)

摘要/Abstract

摘要： 目的

比较低龄儿童龋患者和健康者唾液生化指标的差异及其与龋病状态的关联，建立龋病诊断模型。

方法

选取4~6岁儿童共120名，分为2组：低龄儿童龋患者（C组）和健康儿童（H组）各60名，采集唾液样本，比较pH、总蛋白以及离子浓度的差异，分析唾液生化指标与龋病状态的相关性，并建立龋病诊断模型。

结果

C组NO₃^-浓度显著低于H组，而Cl^-、Br^-、NH₄⁺、Mg²⁺浓度显著高于H组（P<0.05）；唾液NO₃^-浓度与患龋状态呈负相关，Br^-、Cl^-、NH₄⁺浓度与患龋状态呈正相关（P<0.05）。利用唾液生化参数建立的龋病风险评估模型区分C组和H组的准确率可达85%以上。

结论

唾液pH、总蛋白和离子浓度有助于诊断和评估低龄儿童龋。

关键词: 低龄儿童龋, 唾液, 生化指标, 关联性, 诊断模型

Abstract: Objective

This study aimed to compare the salivary biochemical indices between caries-free individuals and those with early childhood caries (ECC), and construct a saliva-based caries diagnostic model by analyzing the correlation between salivary biochemical indices and caries severity.

Methods

A total of 120 children aged 4-6 years were selected and divided into two groups: individuals with ECC (C group, n=60) and healthy children (H group, n=60). Salivary samples were collected to compare the pH, total protein, and ion concentrations between the two groups. The correlation between the salivary biochemical indices and caries severity was examined, and an ECC diagnostic model was established.

Results

The NO₃^- concentration significantly decreased in the C group, whereas the Cl^-, Br^-, NH₄⁺, and Mg²⁺ concentrations significantly increased in the C group (P<0.05). In addition, the salivary caries severity had a significantly negative correlation with the NO₃^- concentration but had a positive correlation with Br^-, Cl^-, and NH₄⁺ concentrations (P<0.05). The ECC diagnostic model based on salivary biochemical indices could yield satisfactory results in terms of distinguishing the C and H groups with over 85% accuracy.

Conclusion

Salivary biochemical indices can contribute to the diagnosis and risk assessment of ECC.

Key words: early childhood caries, saliva, biochemical indices, correlation, diagnosis model

中图分类号:

R 781.1

张颖, 贾松菠, 李帆, 李姗姗, 张利娟, 谭凯璇, 卢洁, 杨芳. 低龄儿童龋病相关的唾液生化指标研究[J]. 华西口腔医学杂志, 2021, 39(3): 300-305.

Zhang Ying, Jia Songbo, Li Fan, Li Shanshan, Zhang Lijuan, Tan Kaixuan, Lu Jie, Yang Fang. Salivary biochemical indices related to early childhood caries[J]. West China Journal of Stomatology, 2021, 39(3): 300-305.

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唾液参数	C组	H组	P值
NO₃^-/（mg·L^-1）	10.90±13.18	32.93±66.46	***
Cl^-/（mg·L^-1）	599.52±173.05	471.25±100.80	***
NH₄⁺/（mg·L^-1）	197.34±67.21	159.34±56.82	*
Mg²⁺/（mg·L^-1）	3.51±1.98	3.01±2.24	*
Br^-/（mg·L^-1）	2.26±1.47	1.29±0.75	***
NO₂^-/（mg·L^-1）	2.44±3.12	3.48±4.75	NS
总蛋白/（mg·L^-1）	1 173.89±325.35	1 116.75±332.62	NS
K⁺/（mg·L^-1）	785.73±246.77	731.54±188.63	NS
PO₄^3-/（mg·L^-1）	468.48±166.66	421.10±126.18	NS
Na⁺/（mg·L^-1）	100.61±64.77	76.86±29.57	NS
F^-/（mg·L^-1）	32.19±18.05	28.59±14.73	NS
SO₄^2-/（mg·L^-1）	21.96±9.49	18.17±5.92	NS
Ca²⁺/（mg·L^-1）	14.40±6.55	10.92±6.37	NS
pH	6.85±0.20	6.85±0.23	NS

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低龄儿童龋病相关的唾液生化指标研究

Salivary biochemical indices related to early childhood caries

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