Gli1阳性细胞在牙周组织发育中的时空分布特点及功能研究

doi:10.7518/hxkq.2020.02.003

华西口腔医学杂志 ›› 2020, Vol. 38 ›› Issue (2): 128-132.doi: 10.7518/hxkq.2020.02.003

Gli1阳性细胞在牙周组织发育中的时空分布特点及功能研究

王韵¹, 谢旭东², 许春梅², 王骏²()

^1. 口腔疾病研究国家重点实验室国家口腔疾病临床医学研究中心四川大学华西口腔医院,成都 610041
^2. 口腔疾病研究国家重点实验室国家口腔疾病临床医学研究中心四川大学华西口腔医院牙周科,成都 610041

收稿日期:2019-10-15 修回日期:2020-01-08 出版日期:2020-04-01 发布日期:2020-04-15
通讯作者: 王骏 E-mail:junwang@scu.edu.cn
作者简介:王韵,硕士,E-mail：wy630ivy@outlook.com
基金资助:
国家自然科学基金(81700980);四川省科技计划项目(2019YJ0097);中国博士后科学基金(2017M623048);四川大学专职博士后研发基金(2018SCU12018)

Temporal and spatial distribution of Gli1⁺ cells and their function during periodontal development

Wang Yun¹, Xie Xudong², Xu Chunmei², Wang Jun²()

^1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
^2. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Periodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China

Received:2019-10-15 Revised:2020-01-08 Online:2020-04-01 Published:2020-04-15
Contact: Jun Wang E-mail:junwang@scu.edu.cn
Supported by:
The National Natural Science Foundation of China(81700980);Sichuan Province Science and Technology Support Program(2019YJ0097);China Postdoctoral Science Foundation(2017M623048);Postdoctoral Research Foundation of Sichuan University(2018SCU12018)

摘要/Abstract

摘要：

目的利用转基因小鼠探究牙周膜内Gli 阳性（Gli1 ⁺）细胞的表达分布以及Gli1 ⁺细胞在牙周组织发育过程中的作用。方法收集3、6和8周龄Gli1 ^lacZ/+小鼠下颌骨,通过β-半乳糖苷酶组织化学染色（X-gal染色）观察牙周膜内Gli1 ⁺细胞的时间和空间分布特点。然后,通过注射他莫昔芬诱导3周龄Gli1-Cre ^ERT2/+;R26R ^tdTomato/+小鼠表达红色荧光蛋白tdTomato,对Gli1 ⁺细胞及其子代细胞（tdTomato ⁺细胞）进行动态追踪。结果 3周龄小鼠牙周膜内分布大量Gli1 ⁺细胞,随着年龄的增长,Gli1在牙周膜内的数量逐渐减少（P<0.05）。tdTomato ⁺细胞随着诱导时间的延长,数量逐渐增加（P<0.05）,且逐渐分化成熟为成纤维细胞、牙骨质细胞和骨细胞。结论牙周膜内Gli1 ⁺细胞具有多向分化的潜能,是牙周组织发育过程中重要的细胞来源。

关键词: Gli1, 牙周组织, 发育, 谱系示踪, 干细胞

Abstract:

Objective This study aimed to investigate the distribution of Gli1 ⁺ cells in the periodontal ligament (PDL) and to evaluate their contribution in the development of periodontal tissue by using transgenic mouse lines. Methods Gli1 ^lacZ/+ mice were harvested at different ages (3, 6, and 8 weeks), and the temporal and spatial distribution patterns of Gli1 ⁺PDL cells were revealed by X-gal staining. Afterward, 3-week-old Gli1-Cre ^ERT2/+;R26R ^tdTomato/+mice were administered with tamoxifen, and the fates of Gli1 ⁺cells and their descendants were traced during periodontal development. Results A large number of Gli1 ⁺cells were detected in the PDL of the 3-week-old mice; however, their number significantly decreased from 3 weeks to 8 weeks (P<0.05). Cell lineage tracing data showed that the descendants of Gli1 ⁺ cells dramatically increased from 3 weeks to 8 weeks (P<0.05) and gradually differentiated into fibroblasts, cementocytes, and osteocytes. Conclusion The multi-differentiation potential of Gli1 ⁺PDL cells was revealed, indicating that Gli1 ⁺cells are an important cell source for periodontal development.

Key words: Gli1, periodontal tissue, development, lineage tracing, stem cell

中图分类号:

Q254

王韵, 谢旭东, 许春梅, 王骏. Gli1阳性细胞在牙周组织发育中的时空分布特点及功能研究[J]. 华西口腔医学杂志, 2020, 38(2): 128-132.

Wang Yun, Xie Xudong, Xu Chunmei, Wang Jun. Temporal and spatial distribution of Gli1⁺ cells and their function during periodontal development[J]. West China Journal of Stomatology, 2020, 38(2): 128-132.

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Gli1阳性细胞在牙周组织发育中的时空分布特点及功能研究

Temporal and spatial distribution of Gli1⁺ cells and their function during periodontal development

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Gli1阳性细胞在牙周组织发育中的时空分布特点及功能研究

Temporal and spatial distribution of Gli1+ cells and their function during periodontal development

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Temporal and spatial distribution of Gli1⁺ cells and their function during periodontal development