microRNA-125b在舌鳞状细胞癌中的表达及意义

doi:10.7518/hxkq.2020.01.003

摘要/Abstract

摘要：

目的检测舌鳞状细胞癌（TSCC）中microRNA-125b的表达,分析与TSCC患者临床病理特征的关系。方法采用实时荧光定量聚合酶链反应（RT-qPCR）检测35例TSCC组织及对应的癌旁正常组织中microRNA-125b的表达情况,并分析microRNA-125b在TSCC组织中的表达与临床特征的关系。采用原位杂交技术（ISH）检测35例TSCC组织及对应的癌旁正常组织切片中microRNA-125b基因的表达水平。结果 RT-qPCR结果显示,TSCC组织和癌旁正常组织中microRNA-125b的相对表达量分别为2.32±0.69和0.87±0.32,TSCC组织中microRNA-125b的表达显著高于癌旁正常组织,差异有统计学意义（P<0.001）;35例TSCC组织中microRNA-125b的表达水平与年龄、性别、病理分级和淋巴结转移无明显相关性,而与TNM分期有明显相关性,分期越高microRNA-125b的表达水平越高（P<0.05）。经过microRNA-125b探针杂交染色结果显示,35例患者的TSCC组织和癌旁正常组织的染色平均密度分别为0.010±0.003和0.004±0.001,癌组织高于癌旁组织,差异有统计学意义（P<0.05）。结论 microRNA-125b在35例TSCC患者中高表达,并与临床分期相关,提示microRNA-125b表达上调可能参与TSCC的发生发展。

关键词: microRNA-125b, 舌鳞状细胞癌, 实时荧光定量聚合酶链反应, 原位杂交

Abstract:

Objective The expression of microRNA-125b in tongue squamous cell carcinoma (TSCC) was detected and analyzed for its relationship with the clinicopathological features of TSCC. Methods Real time fluorescence-quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of microRNA-125b in 35 TSCC tissues and adjacent normal tissues from 35 TSCC cases. The relationship between the expression of microRNA-125b in TSCC tissues and the clinicopathological features of patients with TSCC was analyzed. In situ hybridization (ISH) was used to detect the expression level of microRNA-125b gene in the TSCC tissues and adjacent normal tissues. Results RT-qPCR results showed that the relative expression levels of microRNA-125b in the TSCC issues was 2.32±0.69, and that of normal tissues was 0.87±0.32. The statistical results showed that the expression level of microRNA-125b was significantly higher in the TSCC tissues than in the normal tissues (P<0.001). The expression level of microRNA-125b in the TSCC tissues was not significantly correlated with age, gender, pathological grade, and lymph node metastasis but was positively correlated with TNM stage. Patients with high TNM stage had high microRNA-125b expression levels (P<0.05). The ISH results showed that the expression levels of microRNA-125b in the TSCC tissues were 0.010±0.003, and that of normal tissues was 0.004±0.001. The expression levels of microRNA-125b in the 35 TSCC tissues were significantly higher than those in the normal tissues (P<0.05). Conclusion MicroRNA-125b is highly expressed in TSCC and associated with TNM stage, suggesting that high microRNA-125b expression may be involved in the development of TSCC.

Key words: microRNA-125b, tongue squamous cell carcinoma, real time fluorescence-quantitative polymerase chain reaction, in situ hybridization

中图分类号:

R739.86

王健,闫广鹏,郭超,李军. microRNA-125b在舌鳞状细胞癌中的表达及意义[J]. 华西口腔医学杂志, 2020, 38(1): 11-16.

Wang Jian,Yan Guangpeng,Guo Chao,Li Jun. Expression and significance of microRNA-125b in tongue squamous cell carcinoma[J]. West China Journal of Stomatology, 2020, 38(1): 11-16.

图/表 2

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临床特征		例数	TSCC表达量	统计量	P值
年龄/岁		57.86±6.29^＃	2.32±0.69	-0.157^Δ	0.367
性别	男	10	2.34±0.90	0.261	0.796
	女	25	2.26±0.82
淋巴结转移	有	24	2.41±0.82	0.401	0.691
	无	11	2.28±0.91
TNM分期	Ⅰ+Ⅱ	14	1.88±0.76	2.615	0.013*
	Ⅲ+Ⅳ	21	2.61±0.83
组织分化	低	9	2.28±0.85	0.044^ΔΔ	0.957
程度	中	10	2.28±0.89
	高	16	2.37±0.92