华西口腔医学杂志 ›› 2019, Vol. 37 ›› Issue (3): 253-259.doi: 10.7518/hxkq.2019.03.005

• 基础研究 • 上一篇    下一篇

软骨组织工程用羧甲基壳聚糖/氧化海藻酸钠复合水凝胶的制备及体外评估

李云洁1,滕彬宏1,赵艳红1(),杨强2,王连永3,黄颖1   

  1. 1.天津医科大学口腔医院正畸科,天津 300070
    2.天津医院脊柱外科,天津 300211
    3.南开大学生命科学学院生物活性材料教育部重点实验室,天津 300071
  • 收稿日期:2018-12-15 修回日期:2019-03-10 出版日期:2019-06-01 发布日期:2019-06-12
  • 通讯作者: 赵艳红 E-mail:leafzh@126.com
  • 作者简介:李云洁,硕士,E-mail:250883893@qq.com
  • 基金资助:
    国家自然科学基金(31300798);国家自然科学基金(31470937);国家自然科学基金(81572154);天津市卫生局科技攻关重点项目(15KG125);天津市卫生局科技攻关重点项目(16KG114)

Preparation and evaluation of carboxymethyl chitosan/sodium alginate hydrogel for cartilage tissue engineering

Yunjie Li1,Binhong Teng1,Yanhong Zhao1(),Qiang Yang2,Lianyong Wang3,Ying Huang1   

  1. 1.Dept. of Orthodontics, Stomatological Hospital of Tianjin Medical University, Tianjin 300070, China
    2.Dept. of Spine Surgery, Tianjin Hospital, Tianjin 300211, China
    3.The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China
  • Received:2018-12-15 Revised:2019-03-10 Online:2019-06-01 Published:2019-06-12
  • Contact: Yanhong Zhao E-mail:leafzh@126.com
  • Supported by:
    The National Science Foundation of China(31300798);The National Science Foundation of China(31470937);The National Science Foundation of China(81572154);The Research Foundation of Tianjin Health Bureau(15KG125);The Research Foundation of Tianjin Health Bureau(16KG114)

摘要:

目的 优化羧甲基壳聚糖/氧化海藻酸钠(CMCS/OSA)复合水凝胶制备工艺,并应用于软骨组织工程。方法 制备氨基与醛基比例分别为2:1、1:1、1:2的CMCS/OSA复合水凝胶,并通过扫描电子显微镜观察、流变学检测、黏附拉力测试、溶胀率分析及细胞实验等方法评价复合水凝胶的微观形态、物理特性以及生物相容性,以制备满足软骨再生领域需求的水凝胶。结果 氨基与醛基比为1:1时,制备出的水凝胶具有良好的孔隙率、适宜的成胶时间、较强的黏附力及稳定的溶胀率等性质,对细胞有良好的生物相容性。结论 氨基与醛基比为1:1时制备出的CMCS/OSA复合水凝胶是软骨组织工程优异的支架材料。

关键词: 羧甲基壳聚糖, 氧化海藻酸钠, 水凝胶, 脐带间充质干细胞, 软骨组织工程

Abstract:

Objective This study aimed to optimize the preparation of carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels. This study also aimed to investigate the applicability of the hydrogels in cartilage tissue engineering. Methods Three groups of CMCS/OSA composite hydrogels with amino-to-aldehyde ratios of 2:1, 1:1 and 1:2 were prepared. The microstructure, physical properties, and cell biocompatibility of the three groups of CMCS/OSA composite hydrogels were evaluated. Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering. Results The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1:1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility. Conclusion The CMCS/OSA compound hydrogel prepared with a 1:1 ratio of amino and aldehyde groups has potential applications in cartilage tissue engineering.

Key words: carboxymethyl chitosan, sodium alginate, hydrogel, umbilical cord mesenchymalstem cell, cartilage tissue engineering

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