华西口腔医学杂志 ›› 2018, Vol. 36 ›› Issue (6): 602-608.doi: 10.7518/hxkq.2018.06.004

• 基础研究 • 上一篇    下一篇

钙离子对人成骨细胞迁移与成骨分化的影响

雷群,林东,黄文秀,吴东,陈江()   

  1. 福建医科大学附属口腔医院,福州 350000
  • 收稿日期:2018-01-12 修回日期:2018-09-23 出版日期:2018-12-01 发布日期:2018-12-12
  • 通讯作者: 陈江 E-mail:dentistjiangchen@163.com
  • 作者简介:雷群,主治医师,博士,E-mail: 1467357037@qq.com
  • 基金资助:
    国家科技支撑计划(2012BAI17B03-6);福建省卫计委青年基金(2015-1-66);福建省口腔医院博士科研基金(2015-KQ-YY-BS-6)

Effects of calcium ion on the migration and osteogenic differentiation of human osteoblasts

Qun Lei,Dong Lin,Wenxiu Huang,Dong Wu,Jiang Chen()   

  1. Stomatological Hospital of Fujian Medical University, Fuzhou 350000, China
  • Received:2018-01-12 Revised:2018-09-23 Online:2018-12-01 Published:2018-12-12
  • Contact: Jiang Chen E-mail:dentistjiangchen@163.com
  • Supported by:
    National Science and Technology Support Program(2012BAI17B03-6);Youth Found of Fujian Provincial Health and Family Planning Commission(2015-1-66);Doctoral Research Fund from Stomatological Hospital of Fujian Medical University(2015-KQ-YY-BS-6)

摘要:

目的 研究不同浓度Ca 2+对人成骨细胞迁移与成骨分化的影响,探讨促进迁移与成骨合适的Ca 2+浓度及相关机制。 方法 设置Ca 2+浓度,Transwell检测成骨细胞迁移;CCK-8法评估成骨细胞增殖;逆转录聚合酶链反应(RT-PCR)检测细胞成骨分化相关基因的表达;茜素红染色检测成骨分化生成的矿化结节。钙敏感受体(CaSR)拮抗剂拮抗后,观察Ca 2+对人成骨细胞迁移与成骨分化的影响。 结果 在迁移实验中,2、4、6 mmol·L -1的Ca 2+在3个时间点(8、16、24 h)都能明显地促进人成骨细胞的迁移,10 mmol·L -1的Ca 2+在8 h时明显抑制迁移。2~10 mmol·L -1 Ca 2+能促进人成骨细胞的增殖、成骨分化与矿化,8、10 mmol·L -1的Ca 2+诱导的矿化作用更明显。CaSR拮抗降低Ca 2+诱导的人成骨细胞迁移与成骨分化作用。 结论 低浓度Ca 2+有利于人成骨细胞迁移,高浓度Ca 2+有利于人成骨细胞分化,4、6 mmol·L -1的Ca 2+能较明显地同时诱导人成骨细胞迁移与成骨分化,Ca 2+-CaSR通路参与相应的信号传导。

关键词: 钙离子, 人成骨细胞, 迁移, 钙敏感受体, 骨替代材料

Abstract:

Objective This study aimed to investigate the effect of calcium ion (Ca 2+) on the migration and osteogenic differentiation of human osteoblasts and explore the proper concentration and correlation mechanism. Methods A series of Ca 2+solutions with different concentrations was prepared. Osteoblast migration was assessed by Transwell assay, and proli-feration was studied via the CCK-8 colorimetric assay. The mRNA expression of osteogenic genes was examined via reverse transcription-polymerase chain reaction (RT-PCR), and the mineralized nodule was examined by alizarin red-S method. After calcium sensitive receptor (CaSR) antagonism, Ca 2+-induced migration and osteogenic differentiation were analyzed. Results In the migration experiment, 2, 4, and 6 mmol·L -1Ca 2+could promoted osteoblast migration at three timepoints (8, 16, and 24 h), whereas 10 mmol·L -1 Ca 2+considerably inhibited migration at 8 h. The Ca 2+ concentration range of 2-10 mmol·L -1 could promote proliferation, osteogenic differentiation, and mineralization of human osteoblasts. Moreover, mineralization was predominantly induced by 8 and 10 mmol·L -1Ca 2+. CaSR antagonism could reduce Ca 2+-induced migration and osteogenic differentiation of human osteoblasts. Conclusion Low Ca 2+ concentration favored osteoblast migration, whereas high Ca 2+ concentration favored osteogenic differentiation. The Ca 2+ concentrations of 4 and 6 mmol·L -1 could substantially induce osteo-blast migration and osteogenic differentiation, and the Ca 2+-CaSR pathway participated in signal transduction.

Key words: calcium ion, human osteoblast, migration, calcium sensitive receptor, bone substitute

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