抑菌浓度米诺环素对成骨细胞增殖、分化和矿化的影响

doi:10.7518/hxkq.2018.02.005

华西口腔医学杂志 ›› 2018, Vol. 36 ›› Issue (2): 140-145.doi: 10.7518/hxkq.2018.02.005

抑菌浓度米诺环素对成骨细胞增殖、分化和矿化的影响

邵华英^1,^2,³(), 张一弓^1,^2,³, 杨雪^1,^2,³, 张琼月^1,^2,³, 吴小红^1,³

1.重庆医科大学附属口腔医院修复科
2.口腔疾病与生物医学重庆市重点实验室
3.重庆市高校市级口腔生物医学工程重点实验室,重庆 401147

收稿日期:2017-10-24 修回日期:2018-01-25 出版日期:2018-04-10 发布日期:2018-04-10
作者简介:
邵华英,硕士,E-mail：452429167@qq.com
基金资助:
国家自然科学基金（81200767,H1402）;重庆市基础科学与前沿技术研究专项资助（cstc2017jcyjAX0215）;重庆市渝中区科技计划项目（20170117）;重庆市渝北区科技计划项目（渝北财签[2011]102号）;2016年重庆高校创新团队建设计划资助项目（CXTDG201602006）

Effects of inhibitory concentration minocycline on the proliferation, differentiation, and mineralization of osteoblasts

Huaying Shao^1,^2,³(), Yigong Zhang^1,^2,³, Xue Yang^1,^2,³, Qiongyue Zhang^1,^2,³, Xiaohong Wu^1,³

1. Dept. of Prosthodontics, Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 401147, China
2. Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, China
3. Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China

Received:2017-10-24 Revised:2018-01-25 Online:2018-04-10 Published:2018-04-10
Supported by:
Supported by: The National Natural Science Foundation of China (81200767, H1402);Chongqing Research Program of Basic Research and Frontier Technology (cstc2017jcyjAX0215);Science and Technology Planning Project of Chongqing Yuzhong (20170117);Science and Technology Planning Project of Chongqing Yubei ([2011]102);Program for Innovation Team Building at Institutions of Higher Education in Chongqing in 2016 (CXTDG201602006).

摘要/Abstract

摘要：

目的研究抑菌浓度的米诺环素对成骨细胞增殖、分化和矿化及Runt相关转录因子2（Runx2）、碱性磷酸酶（ALP）、骨桥蛋白（OPN）mRNA表达的影响。方法将米诺环素溶液（0、0.1、0.5、1、10 μg·mL^-1）与原代成骨细胞共培养,CCK-8检测增殖活性,通过ALP活性检测、茜素红染色、荧光定量聚合酶链反应检测探讨米诺环素对成骨细胞分化、矿化的影响。结果 0.1、0.5、1 μg·mL^-1的米诺环素可以促进细胞的增殖,上调ALP、Runx2 mRNA的表达水平,增加钙含量及钙化结节的形成,其中1 μg·mL^-1具有最大促进作用（P<0.05）;当浓度为10 μg·mL^-1时这种促进作用开始下降,并对ALP活性和OPN表达有显著抑制作用（P<0.01）。结论适宜抑菌浓度的米诺环素能促进成骨细胞增殖,上调Runx2、ALP、OPN的表达水平,促进成骨细胞分化和矿化。

关键词: 米诺环素, 成骨细胞, 碱性磷酸酶, 增殖

Abstract:

Objective To study the effect of the inhibitory concentration minocycline on the proliferation, differentiation, and expression of Runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP) and osteopontin (OPN) mRNA of osteoblasts. Methods Primary osteoblasts were cultured in osteogenic induction medium containing 0, 0.1, 0.5, 1, 10 μg·mL^-1minocycline. Cell counting kit-8 was used to observe cell proliferation. ALP activity assay, alizarin red S staining, and real-time quantitative polymerase chain reaction (PCR) were used to determine cell differentiation and mineralization. Results The groups with 0.1, 0.5, 1 μg·mL^-1 minocycline promoted cell proliferation. The mRNA expression levels of ALP and Runx2 were up-regulated. Osteoblast-mediated mineralization was increased. The group with 1 μg·mL^-1 showed maximal promotion effect (P<0.05). When the concentration increased to 10 μg·mL^-1, the promoting effect began to decline, and the ALP activity and OPN expression were significantly inhibited (P<0.01). Conclusion Appropriate concentration of minocycline can promote osteoblasts proliferation, up-regulate the expression levels of Runx2, ALP and OPN, and increase the differen-tiation and mineralization of osteoblasts.

Key words: minocycline, osteoblast, alkaline phosphatase, proliferation

中图分类号:

R781.05

邵华英, 张一弓, 杨雪, 张琼月, 吴小红. 抑菌浓度米诺环素对成骨细胞增殖、分化和矿化的影响[J]. 华西口腔医学杂志, 2018, 36(2): 140-145.

Huaying Shao, Yigong Zhang, Xue Yang, Qiongyue Zhang, Xiaohong Wu. Effects of inhibitory concentration minocycline on the proliferation, differentiation, and mineralization of osteoblasts[J]. West China Journal of Stomatology, 2018, 36(2): 140-145.

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抑菌浓度米诺环素对成骨细胞增殖、分化和矿化的影响

Effects of inhibitory concentration minocycline on the proliferation, differentiation, and mineralization of osteoblasts

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