华西口腔医学杂志

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人工合成抗菌肽对口腔细菌抗菌性能的初步研究

刘奕1 费伟1 王丽娜2 董广艳3 吴红崑3   

  1. 1.四川省医学科学院•四川省人民医院口腔科,成都 610072;2.大连医科大学口腔医院口腔内科,大连 116044;3.口腔疾病研究国家重点实验室 华西口腔医院老年口腔科(四川大学),成都 610041
  • 出版日期:2014-12-01 发布日期:2014-12-01
  • 通讯作者: 吴红崑,教授,博士,E-mail:whk-mo@263.net
  • 作者简介:刘奕,主治医师,博士,E-mail:liuyi82404@hotmail.com
  • 基金资助:

    四川省科技支撑计划基金资助项目(2008FZ0173)

Antibacterial activity of synthetic antimicrobial decapeptide against oral bacteria

 Liu Yi1, Fei Wei1, Wang Lina2, Dong Guangyan3, Wu Hongkun3.   

  1. 1. Dept. of Oral Medicine, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, Chengdu 610072, China; 2. Dept. of Oral Medicine, School of Stomatology, Dalian Medical University, Dalian 116044, China; 3. State Key Laboratory of Oral Diseases, Dept. of Geriatric Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Online:2014-12-01 Published:2014-12-01

摘要:

目的 评价人工合成抗菌肽(十肽)对口腔常见感染性疾病主要致病菌的抑菌活性。方法 采用琼脂扩散法及液体稀释法体外评价十肽对变异链球菌、表兄链球菌、嗜酸乳杆菌、血链球菌、格氏链球菌、黏性放线菌、内氏放线菌、牙龈卟啉单胞菌、中间普雷沃菌、具核梭杆菌、伴放线嗜血杆菌及白色假丝酵母菌的抑菌性能,并测定十肽对变异链球菌的时间-杀菌曲线。结果 十肽对所选实验菌株均表现出不同的抑菌性能,对主要致龋菌的最小抑菌浓度MIC值为62.5~125 μg·mL-1,而对主要牙周致病菌的MIC值为250~1 000 μg·mL-1,其中,十肽对龋病主要致病菌变异链球菌有较强抑菌作用。时间-杀菌曲线结果显示,十肽作用20 min后开始杀菌,30 min之后可完全杀灭细菌,且在24 h之内无细菌生长。结论 新型人工合成抗菌肽十肽对口腔常见感染性疾病主要致病菌具有抑菌性能,其中对致龋变异链球菌的抑菌效果最为明显。

关键词: 十肽, 抗菌性能, 口腔感染性疾病

Abstract:

Objective This study aims to evaluate the antimicrobial activity of decapeptide, a novel antimicrobial peptide, against several major cariogenic and periodontopathogenic bacteria in vitro. Methods In this study, we investigated the antimicrobial activity of decapeptide against Streptococcus mutans (S. mutans), Streptococcus sobrinus, Lactobacillus acidophilus, Streptococcus sanguis, Streptococcus gordonii, Actinomyces viscosus, Actinomyces naeslundii, Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans, and Saccharomyces albicans in vitro using the agar diffusion method and broth dilution method. Furthermore, a time–kill kinetic study of decapeptide against S. mutans was performed. Results The results showed that decapeptide exhibited antimicrobial activity against various oral bacteria and fungi. The minimum inhibitory concentration (MIC) of main cariogenic bacteria ranged from 62.5 μg·mL-1 to 125 μg·mL-1, and the MIC of periodontopathogenic bacteria tested ranged from 250 μg·mL-1 to 1 000 μg·mL-1. Among the bacteria tested, decapeptide had a strong inhibitory effect on cariogenic S. mutans. Results of the time–kill kinetic studies showed that decapeptide reduced the viable counts of S. mutans by more than one order of magnitude after 20 min of incubation, and thoroughly killed S. mutans after 30 min. No viable cells could be detected after 24 h of incubation. Conclusion This study suggest that decapeptide might have potential clinical application in treating dental caries by killing S. mutans within dental plaque.

Key words: decapeptide, antibacterial activity, oral infectious disease