华西口腔医学杂志

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载银抗菌纯钛表面的制备及其抗菌性能的检测

廖娟1 费伟1 郭俊1 李鹏2   

  1. 1.四川省医学科学院•四川省人民医院口腔科;2.麻醉科,成都 610072
  • 出版日期:2014-06-01 发布日期:2014-06-01
  • 通讯作者: 李鹏,主治医师,硕士,E-mail:lipengmazui@qq.com
  • 作者简介:廖娟,主治医师,博士,E-mail:juan123liao@163.com

Preparation and anti-bacterial tests of silver-modified titanium surface

Liao Juan1, Fei Wei1, Guo Jun1, Li Peng2   

  1. 1. Dept. of Stomatology, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, Chengdu 610072, China; 2. Dept. of Anesthesiology, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, Chengdu 610072, China
  • Online:2014-06-01 Published:2014-06-01

摘要:

目的  对纯钛表面进行抗菌改性,以降低纯钛种植体周围炎的发生率,从而提高种植术的成功率。方法 采用水相硅烷化方法,将3-(氨基丙基)三乙氧基硅烷(APS)组装在湿化学法处理的钛表面上,并将纳米银被覆在该表面上,采用X射线光电子能谱(XPS)和X射线能谱(EDX)对该改性表面理化性能进行分析,观察其对金黄色葡萄球菌和大肠杆菌的抗菌性能。结果 扫描电镜观察可见,改性后的钛表面有不规则纳米银颗粒散在的附着在其表面上,XPS分析显示银的含量为6.8%。经过24 h需氧培养,纳米银改性钛片对金黄色葡萄球菌的抗菌率达到94.23%,对大肠杆菌的抗菌率达95.34%。结论 经过纳米银改性后的钛片具有抗菌性能。

关键词: 钛, 纳米银, 抗菌性能, 水相硅烷化

Abstract:

Objective  To deposit silver nanoparticles on a titanium surface to obtain antibacterial properties. To reduce the incidence of peri-implantitis, and improve the success rate of implantation. Methods  A silver nanoparticle-modified titanium (Ti-nAg) surface was prepared using silanization method, and its surface was characterized by using X-ray photoelec-tron spectroscopy (XPS) and energy dispersive X-ray spectrometer (EDX). Two species of bacteria, namely, Staphylococcus aureus and Escherichia coli, were used to test the antibacterial effect of Ti-nAg surface. Results  Scanning electron micro-scope (SEM) revealed that a small quantity of silver nanoparticles were deposited on the titanium surface. XPS analyses revealed that 6.8% of silver was present on the titanium surface. After 24 h of incubation, 94.23% of Staphylococcus aureus and 95.34% of Escherichia coli were killed on the Ti-nAg surface. Conclusion  Results suggest that silver nanoparticle-modified titanium is a promising material with an antibacterial property that may be used as an implantable biomaterial.

Key words: titanium, nano-silver, antibacterial activity, silanization method