关键词: 脂多糖, 人牙周膜干细胞, 炎性因子

Abstract:

Objective  To investigate the effect of Porphyromonas gingivalis lipopolysaccharide（LPS） on proliferation and inflammatory factors expression of human periodontal ligament stem cells（HPDLSCs）. Methods  HPDLSCs were cultivated and identified. Experiment was divided into 3 groups according to culture solution: Group A with α-MEM culture solution containing 10 μg•mL^-1 LPS, group B with supernatant fluid containing 10 ng•mL^-1 LPS stimulated mono-cyte, group C with α-MEM culture solution. The proliferation ability of HPDLSCs was analyzed by MTT assay. The ex-pression levels of interleukin-1β（IL-1β）, interleukin-6（IL-6）, tumor necrosis factor（TNF-α） mRNA of HPDLSCs were detected by reverse transcriptase polymerase chain reaction（RT-PCR）. Results  HPDLSCs had clonality, bone and fat differentiation ability. Compared with group C, the proliferation ability of HPDLSCs of group A and group B was sig-nificantly inhibited, and the proliferation ability of HPDLSCs of group B were more significantly inhibited than that of group A（P<0.05）. The expression of IL-1β, IL-6 and TNF-α mRNA of group A and group B increased compared with the control group, and the expression of IL-1β, IL-6 and TNF-α mRNA of group B increased more than that of group A（P<0.05）. Conclusion    Porphyromonas gingivalis may inhibit the proliferation of HPDLSCs directly or indirectly through LPS and increase expression of inflammatory factor, exacerbate periodontal inflammatory tissue damage and delay the self-repairing of periodontal tissue.

Key words: lipopolysaccharide, human periodontal ligament stem cells, inflammatory factor