华西口腔医学杂志 ›› 2012, Vol. 30 ›› Issue (6): 628-631.doi: 10.3969/j.issn.1000-1182.2012.06.016

• 专栏论著 • 上一篇    下一篇

内皮细胞型一氧化氮合成酶Glu298Asp基因多态性与牙周炎伴Ⅱ型糖尿病的相关性研究

侯海娟1 张帆2 赵美林1 曹桂芬3 黄海云4 黄萍4   

  1. 1.重庆医科大学附属口腔医院牙周科； 重庆市口腔疾病与生物医学研究中心， 重庆401147； 2.四川大学华西医院体检中心， 成都610041； 3.浙江大学医学院附属口腔医院牙周科， 杭州310006； 4.四川大学华西口腔医院牙周科， 成都610041
  • 收稿日期:2012-03-26 修回日期:2012-09-23 出版日期:2012-12-01 发布日期:2012-12-01
  • 通讯作者: 黄萍，Tel：028-85501445
  • 作者简介:侯海娟（1984—），女，山东人，硕士
  • 基金资助:

    “十一五”国家科技攻关计划基金资助项目（2007BAI- 18B02）

Relationship between endothelial nitric oxide synthase Glu298Asp gene polymorphisms and the chronic periodontitis with type 2 diabetes mellitus

Hou Haijuan1, Zhang Fan2, Zhao Meilin1, Cao Guifen3, Huang Haiyun4, Huang Ping4.   

  1. 1. Dept. of Periodontology, The Affiliated Hospital of Stomatology, Chongqing Medical University; Chongqing Research Center for Oral Diseases and Biomedical Science, Chongqing 401147, China; 2. Dept. of Medical Examination Center, West China Hospital, Sichuan University, Chengdu 610041, China; 3. Dept. of Periodontology, The Affiliated Stomatological Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, China; 4. Dept. of Periodontology, West China School of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2012-03-26 Revised:2012-09-23 Online:2012-12-01 Published:2012-12-01

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摘要：

目的研究内皮细胞型一氧化氮合成酶（eNOS）Glu298Asp基因多态性与牙周炎伴Ⅱ型糖尿病易感性的关系。方法收集慢性牙周炎患者、慢性牙周炎伴Ⅱ型糖尿病患者、Ⅱ型糖尿病患者和牙周健康者的颊黏膜拭子，提取DNA后应用聚合酶链反应-限制性片段长度多态性法检测eNOS Glu298Asp的基因型分布。结果慢性牙周炎组、 Ⅱ型糖尿病组、慢性牙周炎伴Ⅱ型糖尿病组、正常组eNOS Glu298Asp基因型的分布差异有统计学意义（掊2＝18.503， P＝0.005），等位基因频率分布差异也有统计学意义（掊2＝8.243，P＝0.041）。慢性牙周炎伴Ⅱ型糖尿病组与正常组相比，T基因型的OR值为0.962，95%可信区间为0.737～1.256，说明T基因型可能为糖尿病和牙周炎的保护因素；G基因型的OR值为1.043，95%可信区间为0.781～1.391，说明G基因型可能为二者的危险因素，但差异无统计学意义。结论慢性牙周炎、慢性牙周炎伴Ⅱ型糖尿病、糖尿病的易感性可能与eNOS Glu298Asp多态性有关。

关键词: 慢性牙周炎, Ⅱ型糖尿病, 一氧化氮合成酶, 基因多态性

Abstract:

Objective To study the relationship between endothelial nitric oxide synthase（eNOS） Glu298Asp gene polymorphism and chronic periodontitis（CP） with type 2 diabetes mellitus（T2DM）. Methods DNA from patients’buccal swabs of CP, CP with T2DM, T2DM and health was isolated and extracted. The eNOS Glu298Asp gene polymorphism were assessed by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） respectively. Results The distribution of eNOS Glu298Asp genotype in CP group, T2DM group, CP with T2DM group and health group showed statistical differences（掊2＝18.503, P＝0.005）, and the gene frequency showed statistical differences（掊2= 8.243, P=0.041）. Compared health group to CP with T2DM group, OR value of the genetype T was 0.962, 95% confidence interval lay in between 0.737 to 1.256, which showed T maybe a protective factor. While OR value of the genetype G was 1.043, 95% confidence interval lay in between 0.781 to 1.391, which showed G maybe a risk factor. However, neither T nor G was statistically significant. Conclusion Based on these findings, there are some association between eNOS Glu298Asp polymorphism and the risk of CP group, T2DM group, CP with T2DM group.

Key words: chronic periodontitis, type 2 diabetes mellitus, nitric oxide synthase, gene polymorphism

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