华西口腔医学杂志

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载银纳米二氧化钛树脂基托对变异链球菌和白色假丝酵母菌抗菌性的体外研究

刘杰1 葛亚丽1 徐连立2   

  1. 1.佳木斯大学附属口腔医院修复特诊科; 2.技工加工中心, 佳木斯154002
  • 收稿日期:2012-04-25 修回日期:2012-04-25 出版日期:2012-04-01 发布日期:2012-04-01
  • 通讯作者: 刘杰,Tel:13555596888
  • 作者简介:刘杰(1963—),女,山东人,副教授,博士
  • 基金资助:

    黑龙江省研究生创新科研基金资助项目(YJSCX2011-388-HLJ)

Study of antibacterial effect of polymethyl methacrylate resin base containing Ag-TiO2 against Streptococcus mutans and Saccharomyces albicans in vitro

Liu Jie1, Ge Yali1, Xu Lianli2   

  1. 1. Dept. of Prosthodontics, School of Stomatology, Jiamusi University, Jiamusi 154002, China; 2. Dept. of Denture Production, School of Stomatology, Jiamusi University, Jiamusi 154002, China
  • Received:2012-04-25 Revised:2012-04-25 Online:2012-04-01 Published:2012-04-01
  • Contact: Liu Jie,Tel:13555596888
  • About author:Liu Jie(1963—),女,山东人,副教授,博士

摘要:

目的采用体外法研究载银纳米二氧化钛(Ag-TiO2)树脂基托对口腔常见条件致病菌的单一游离菌体及菌斑生物膜的抗菌性能。方法将不同质量分数的Ag-TiO2抗菌粉剂添加到树脂基托材料中,制成抗菌树脂基托。采用薄膜密贴法分别检测该抗菌树脂基托对变异链球菌、白色假丝酵母菌、金黄色葡萄球菌的抗菌率,并用扫描电镜观察抗菌树脂基托表面变异链球菌、白色假丝酵母菌的生长情况。结果与未添加抗菌剂相比,抗菌剂质量分数为0.7%时,抗菌树脂基托对金黄色葡萄球菌的抗菌率达93.3%;抗菌剂质量分数为1.5%时,抗菌树脂基托对变异链球菌的抗菌率达90.2%;抗菌剂质量分数为2.5%时,抗菌树脂基托对白色假丝酵母菌的抗菌率也达91.2%。与未添加抗菌剂相比,抗菌树脂基托表面变异链球菌、白色假丝酵母菌的黏附数量明显减少。结论Ag-TiO2可以显著提高树脂基托的抗菌性能。

关键词: 载银纳米二氧化钛树脂基托, 抗菌性能, 菌斑

Abstract:

Objective To study the antibacterial effect of polymethyl methyacrylate(PMMA) resin base containing Ag-TiO2 antibacterial agent against common conditioned pathogen and their bacterial plaque in vitro. Methods Different qualities of Ag-TiO2 antibacterial agent were added into PMMA resin base in order to prepare antibacterial PMMA resin base. Then, in vitro analysis of antibacterial effect of the resin base against Streptococcus mutans, Staphylococcus aureus, Saccharomyces albicans were assayed with the pellicle-sticking method. Furthermore, the growth of Streptococcus mutans and Saccharomyces albicans bacterial plaque on the antibacterial PMMA resin base were examined by scanning electron microscope. Results The inhibition percent for Staphylococcus aureus was 93.3% by antibacterial PMMA resin base with 0.7% Ag-TiO2; for Streptococcus mutans 90.2% with 1.5% Ag-TiO2; for Saccharomyces albicans 91.2% with 2.5% Ag-TiO2. Bacterial plaque of Streptococcus mutans and Saccharomyces albicans on antibacterial resin base were inhibited effectively. Conclusion The antibacterial property of the PMMA resin base is effectively enhanced with Ag-TiO2 antibacterial agent.

Key words: polymethyl methacrylate resin base containing Ag-TiO2, antibacterial effect, bacterial plaque