牙龈卟啉单胞菌上调钙结合蛋白1促进牙龈上皮细胞增殖

doi:10.7518/hxkq.2022.01.014

华西口腔医学杂志 ›› 2022, Vol. 40 ›› Issue (1): 93-99.doi: 10.7518/hxkq.2022.01.014

牙龈卟啉单胞菌上调钙结合蛋白1促进牙龈上皮细胞增殖

张雨薇(), 何雨轩, 丁一, 刘程程()

口腔疾病研究国家重点实验室国家口腔疾病临床医学研究中心四川大学华西口腔医院牙周病科，成都 610041

收稿日期:2021-02-08 修回日期:2021-10-04 出版日期:2022-02-01 发布日期:2022-02-07
通讯作者: 刘程程 E-mail:luckyzhyw@163.com;liuchengcheng519@163.com
作者简介:张雨薇，硕士，E-mail：luckyzhyw@163.com
基金资助:
国家自然科学基金(81600871)

Porphyromonas gingivalis upregulates calbindin 1 and thus promotes the proliferation of gingival epithelial cells

Zhang Yuwei(), He Yuxuan, Ding Yi, Liu Chengcheng.()

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Periodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China

Received:2021-02-08 Revised:2021-10-04 Online:2022-02-01 Published:2022-02-07
Contact: Liu Chengcheng. E-mail:luckyzhyw@163.com;liuchengcheng519@163.com
Supported by:
The National Natural Science Foundation of China(81600871);Correspondence: Liu Chengcheng, E-mail: liuchengcheng519@163.com

摘要/Abstract

摘要： 目的

探究钙结合蛋白1在牙龈卟啉单胞菌（P. gingivalis）影响牙龈上皮细胞增殖和凋亡中的作用。

方法

P. gingivalis感染CA9-22细胞，在感染24 h后，采用实时荧光定量聚合酶链反应、免疫印迹法和免疫荧光法检测钙结合蛋白1（CALB1）的表达。通过RNA干扰法抑制CALB1表达，BrdU分析检测细胞增殖，Caspase 3活性测定检测细胞凋亡，免疫印迹法检测MDM2和p53的表达。

结果

P. gingivalis感染促进CA9-22细胞中CALB1的表达，并且CALB1的表达具有感染复数依赖性。CALB1促进CA9-22细胞增殖，上调细胞内MDM2的表达，同时抑制p53的表达。下调CALB1表达不影响P. gingivalis感染对CA9-22凋亡的抑制作用。

结论

P. gingivalis感染可通过上调CALB1表达促进CA9-22细胞增殖，其机制可能与影响MDM2-p53有关。

关键词: 牙龈卟啉单胞菌, 牙龈上皮细胞, 细胞增殖, 钙结合蛋白1

Abstract: Objective

This study aims to investigate the effect of calbindin 1 on the proliferation and apoptosis of gingival epithelial cells affected by Porphyromonas gingivalis (P. gingivalis)invitro.

Methods

A model of P. gingivalis infecting CA9-22 was established in vitro. At 24 h after infection, the expression of calbindin 1 (CALB1) was detected by real-time fluorescent quantitative polymerase chain reaction, Western blot, and immunofluorescence analyses. The expression of CALB1 was further inhibited by RNA interference. Cell proliferation was detected by BrdU analysis, and cell apoptosis was detected by caspase 3 activity. The expression of MDM2 and p53 was detected by Western blot analysis.

Results

P. gingivalis infection upregulated the expression of CALB1 in CA9-22 cells with multiplicity-dependent manner. CALB1 promoted the proliferation of CA9-22 cells, increased the expression of MDM2, and inhibited the expression of p53. Inhibiting CALB1 expression did not affect the inhibitory effect of P. gingivalis infection on CA9-22 apoptosis.

Conclusion

P. gingivalis infection can promote the proliferation of CA9-22 cells by increasing CALB1 expression. The related mechanism may be associated with MDM2-p53.

Key words: Porphyromonas gingivalis, gingival epithelial cell, cell proliferation, calbindin 1

中图分类号:

R 781.4

张雨薇, 何雨轩, 丁一, 刘程程. 牙龈卟啉单胞菌上调钙结合蛋白1促进牙龈上皮细胞增殖[J]. 华西口腔医学杂志, 2022, 40(1): 93-99.

Zhang Yuwei, He Yuxuan, Ding Yi, Liu Chengcheng.. Porphyromonas gingivalis upregulates calbindin 1 and thus promotes the proliferation of gingival epithelial cells[J]. West China Journal of Stomatology, 2022, 40(1): 93-99.

图/表 4

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牙龈卟啉单胞菌上调钙结合蛋白1促进牙龈上皮细胞增殖

Porphyromonas gingivalis upregulates calbindin 1 and thus promotes the proliferation of gingival epithelial cells

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