关键词: 纯钛, 氧化石墨烯, 工艺表征, 金黄色葡萄球菌, 细胞结构

Abstract:

Objective To evaluate the process characterization of graphene oxide loaded on pure titanium surface and effect on the biological properties of Staphylococcus aureus and osteoblasts. Methods Graphene oxide at four concentrations (20, 50, 80, and 100 μg·mL ^-1) was loaded on the pure titanium surface via electroplating, and the morphology, properties, and hydrophilic properties were measured with a field emission scanning electron microscope, micro Raman spectrometer, and contact angle tester, respectively. In addition, Staphylococcus aureus and osteoblasts were used as models and cultured with pure titanium-graphene oxide. Then, field-emission scanning electron microscopy and laser confocal microscopy were utilized to observe the changes in the amount of bacteria and osteoblast morphology and structure, respectively. Results Graphene oxide at the four concentrations was successfully loaded on pure titanium surface via electroplating. It improved the hydrophilic properties of pure titanium surface, which benefitted the adhesion and growth of Staphylococcus aureus and changed the morphology and structure of the osteoblasts. Conclusion The pure titanium-graphene oxide composite has no antibacterial properties and has good biocompatibility.

Key words: pure titanium, graphene oxide, process characterization, Staphylococcus aureus, cell structure