3D打印多孔钽种植体对骨整合影响的实验研究

doi:10.7518/hxkq.2018.03.012

华西口腔医学杂志 ›› 2018, Vol. 36 ›› Issue (3): 291-295.doi: 10.7518/hxkq.2018.03.012

3D打印多孔钽种植体对骨整合影响的实验研究

苏可欣(), 季平, 王涵, 李林林, 苏雷震, 王超()

重庆医科大学附属口腔医院口腔颌面外科,口腔疾病与生物医学重庆市重点实验室,重庆市高校市级口腔生物医学工程重点实验室,重庆 401147

收稿日期:2017-10-15 修回日期:2018-01-12 出版日期:2018-06-01 发布日期:2018-06-01
作者简介:
苏可欣,住院医师,硕士,E-mail：1026833127@qq.com
基金资助:
国家自然科学基金青年科学基金（11402042）;重庆市基础与前沿研究计划（CSTC2015jcyjA10027）;重庆市科委社会民生一般项目（CSTC2015shmszx10008）;重庆市卫生计生委面上项目（2017MSXMD73）;2016年重庆高校创新团队建设计划（CX-TDG201602006）

In vivo study of 3D printed porous tantalum implant on osseointegration

Kexin Su(), Ping Ji, Han Wang, Linlin Li, Leizhen Su, Chao Wang()

Dept. of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital of Chongqing Medical University, Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China

Received:2017-10-15 Revised:2018-01-12 Online:2018-06-01 Published:2018-06-01
Supported by:
National Natural Science Foundation for Young Scholars of China (11402042);Project of Basic and Frontier Research Plan of Chongqing (CSTC2015jcyjA10027);Social and People’s Livelihood Project of Science and Technology Commission of Chongqing (CSTC2015shmszx10008);Project of Health and Family Plan Commission of Chongqing (2017-MSXMD73);Program for Innovation Team Building at Institutions of Higher Education in Chongqing in 2016 (CXTDG2016-02006).

摘要/Abstract

摘要：

目的研究多孔钽及多孔钛种植体对骨整合的影响。方法通过计算机辅助设计方法建模,采用3D打印技术制备两种微孔参数相同的多孔材料种植体：多孔钽及多孔钛。在24只新西兰大白兔双侧股骨外踝处建立骨缺损模型,每只动物左右侧缺损随机分组,分别用多孔钽（实验组）和多孔钛（对照组）种植体进行修复。种植体植入后2、4、8周取材,进行大体观察和亚甲基蓝-酸性品红染色,观测种植体和骨界面的骨整合情况,采用推出实验测试种植体-骨界面结合强度。结果术后2、4、8周,两组材料界面的新生骨组织逐渐增加,出现新生骨小梁并向材料孔隙内生长;两组的成骨情况及种植体-骨组织界面结合强度的差异无统计学意义（P>0.05）。结论 3D打印的多孔钽能与骨组织形成早期的生物结合,具有与多孔钛相当的骨整合能力。

关键词: 多孔钽, 3D打印, 骨整合, 种植修复

Abstract:

Objective This work aims to investigate the effect of porous tantalum and porous titanium on osseointegration. Methods Two kinds of porous materials with same microporous parameters, namely, porous tantalum and porous titanium, were fabricated by computer-aided design (CAD) modeling and 3D printing technology. A defect model was established in 24 New Zealand white rabbits in the bilateral femoral lateral malleolus at the left and right side of each animal. Then, animals were randomly divided into two groups, and bone defects were repaired by porous tantalum and porous titanium (experimental and control groups, respectively). Animals were sacrificed at two, four, and eight weeks after implantation. Gross observation and methylene blue-acid fuchsin staining were used to observe osseointegration of the implant and bone interface, and the osseointegration strength of implant bone interface was tested by push-out test. Results At two, four, and eight weeks after operation, the new bone tissue in the two groups increased gradually, and new bone trabecula appeared and grew into the pores of the materials. No significant difference (P>0.05) in osteo-genesis and the strength of implant bone tissue interface between the two groups was observed. Conclusion 3D printed porous tantalum implants, which exhibit comparable osseointegration capabilities to porous titanium implants, can form an early biological combination with bone tissue.

Key words: porous tantalum, 3D printing, osseoin-tegration, implant repair

中图分类号:

R783

苏可欣, 季平, 王涵, 李林林, 苏雷震, 王超. 3D打印多孔钽种植体对骨整合影响的实验研究[J]. 华西口腔医学杂志, 2018, 36(3): 291-295.

Kexin Su, Ping Ji, Han Wang, Linlin Li, Leizhen Su, Chao Wang. In vivo study of 3D printed porous tantalum implant on osseointegration[J]. West China Journal of Stomatology, 2018, 36(3): 291-295.

图/表 5

参考文献 15

[1]	Chen L, Dai H, Zhou Y, et al.Porous, single crystalline titanium nitride nanoplates grown on carbon fibers: excellent counter electrodes for low-cost, high performance, fiber-shaped dye-sensitized solar cells[J]. Chem Commun: Camb, 2014, 50(92): 14321-14324.
[2]	刘洪臣, 路荣建. 新型多孔钽人工种植牙[J]. 中华老年口腔医学杂志, 2016, 15(1): 41-44.
	Liu HC, Lu RJ.New type porous tantalum dental implant[J]. Chin J Geriatric Dent, 2016, 15(1): 41-44.
[3]	Lu T, Wen J, Qian S, et al.Enhanced osteointegration on tantalum-implanted polyetheretherketone surface with bone-like elastic modulus[J]. Biomaterials, 2015, 51: 173-183.
[4]	Ryan G, Pandit A, Apatsidis DP.Fabrication methods of porous metals for use in orthopaedic applications[J]. Bio-materials, 2006, 27(13): 2651-2670.
[5]	Liu Y, Bao C, Wismeijer D, et al.The physicochemical/biological properties of porous tantalum and the potential surface modification techniques to improve its clinical app-lication in dental implantology[J]. Mater Sci Eng C Mater Biol Appl, 2015, 49: 323-329.
[6]	Balla VK, Banerjee S, Bose S, et al.Direct laser processing of a tantalum coating on titanium for bone replacement struc-tures[J]. Acta Biomaterialia, 2010(6): 2329-2334.
[7]	Shimko DA, Shimko VF, Sander EA, et al.Effect of poro-sity on the fluid flow characteristics and mechanical pro-perties of tantalum scaffolds[J]. J Biomed Mater Res Part B Appl Biomater, 2005, 73(2): 315-324.
[8]	Karageorgiou V, Kaplan D.Porosity of 3D biomaterial scaffolds and osteogenesis[J]. Biomaterials, 2005, 26(27): 5474-5491.
[9]	Guldberg RE, Duvall CL, Peister A, et al.3D imaging of tissue integration with porous biomaterials[J]. Biomaterials, 2008, 29(28): 3757-3761.
[10]	Rønold HJ, Lyngstadaas SP, Ellingsen JE.Analysing the optimal value for titanium implant roughness in bone attach-ment using a tensile test[J]. Biomaterials, 2003, 24(25): 4559-4564.
[11]	Traini T, Mangano C, Sammons RL, et al.Direct laser metal sintering as a new approach to fabrication of an isoelastic functionally graded material for manufacture of porous tita-nium dental implants[J]. Dent Mater, 2008, 24(11): 1525-1533.
[12]	Zhang Y, Zheng Y, Li Y, et al.Tantalum nitride-decorated titanium with enhanced resistance to microbiologically in-duced corrosion and mechanical property for dental app-lication[J]. PLoS One, 2015, 10(6): e0130774.
[13]	Moroni L, de Wijn JR, van Blitterswijk CA. 3D fiber-depo-sited scaffolds for tissue engineering: influence of pores geometry and architecture on dynamic mechanical properties[J]. Biomaterials, 2006, 27(7): 974-985.
[14]	Xiu P, Jia Z, Lv J, et al.Tailored surface treatment of 3D printed porous Ti6Al4V by microarc oxidation for enhanced osseointegration via optimized bone in-growth patterns and interlocked bone/implant interface[J]. ACS Appl Mater In-terfaces, 2016, 8(28): 17964-17975.
[15]	Inglam S, Chantarapanich N, Suebnukarn S, et al.Biome-chanical evaluation of a novel porous-structure implant: finite element study[J]. Int J Oral Maxillofac Implants, 2013, 28(2): e48-e56.

3D打印多孔钽种植体对骨整合影响的实验研究

In vivo study of 3D printed porous tantalum implant on osseointegration

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 5

参考文献 15

相关文章 15

编辑推荐

Metrics

本文评价

[1]	王旭东, 魏弘朴, 李彪. 从“经验外科”到“精准外科”——精准正颌外科体系的建立与临床应用[J]. 华西口腔医学杂志, 2023, 41(5): 491-501.
[2]	张玲阁, 李洁, 李继宏, 汤宏超, 刘玉学. 射频热凝术联合注射阿霉素治疗三叉神经下颌支痛的临床研究[J]. 华西口腔医学杂志, 2022, 40(4): 446-450.
[3]	贺锦秀, 高静, 刘春煦, 解晨阳, 余嘉怡, 于海洋. 一种序列3D打印导板引导的瓷贴面分区粘接技术[J]. 华西口腔医学杂志, 2022, 40(3): 365-369.
[4]	于海洋. 种植修复里的数字追问——从经验类比到数字引导[J]. 华西口腔医学杂志, 2021, 39(4): 386-397.
[5]	张煜强, 于海洋. 颌间距离还是目标修复空间值——实测数值引导的无牙颌种植修复的临床决策[J]. 华西口腔医学杂志, 2021, 39(2): 233-237.
[6]	刘春煦, 高静, 赵雨薇, 范琳, 贾璐铭, 胡楠, 梅子彧, 董博, 张倩倩, 于海洋. 一种3D打印定深孔导板引导的精准牙体预备技术[J]. 华西口腔医学杂志, 2020, 38(3): 350-355.
[7]	娄方芝, 张茂芮, 饶鹏程, 罗世洪, 唐梦莹, 肖金刚. 数字化导板在前牙美学区种植修复应用的精确度研究[J]. 华西口腔医学杂志, 2020, 38(2): 170-176.
[8]	于文倩,李晓茜,马丽,马晓妮,徐欣. 下颌牙列缺失固定种植修复的颌学研究[J]. 华西口腔医学杂志, 2020, 38(1): 30-36.
[9]	蒋晔,张志宏,刘红红,周航天,芮茜,张莹莹. 3D打印种植导板在前牙区不同术式的精度比较[J]. 华西口腔医学杂志, 2019, 37(4): 403-407.
[10]	潘硕,刘斌. 颅颌面修复体制作用3D打印金属粉末的研究进展[J]. 华西口腔医学杂志, 2019, 37(4): 438-442.
[11]	刘飞,王志峰,刘芳芳,徐巾诏,刘奇博,蓝菁. 微小RNA-29a-3p调节卷曲蛋白4表达影响高脂血症大鼠种植体骨整合的实验研究[J]. 华西口腔医学杂志, 2019, 37(2): 200-207.
[12]	张倩倩,陈昕,赵雨薇,刘春煦,罗天,董博,于海洋. 3D打印在口腔美学修复中的应用[J]. 华西口腔医学杂志, 2018, 36(6): 656-661.
[13]	张玲阁,邓末宏,龙星,王章正. 3D打印导板辅助射频温控热凝术治疗第2支三叉神经痛的临床研究[J]. 华西口腔医学杂志, 2018, 36(6): 662-666.
[14]	罗维佳, 陈莉, 欧国敏. 多数牙先天缺失复杂牙列种植修复方案的设计[J]. 华西口腔医学杂志, 2018, 36(3): 344-347.
[15]	姚洋, 杜宇, 古霞, 光梦凯, 黄波, 宫苹. 局部注射外源性神经生长因子促进小鼠钛种植体周骨胶原早期成熟的研究[J]. 华西口腔医学杂志, 2018, 36(2): 128-132.