丝素蛋白-软骨脱细胞外基质复合取向支架的制备及评价

doi:10.7518/hxkq.2018.01.004

华西口腔医学杂志 ›› 2018, Vol. 36 ›› Issue (1): 17-22.doi: 10.7518/hxkq.2018.01.004

丝素蛋白-软骨脱细胞外基质复合取向支架的制备及评价

滕彬宏¹(), 赵艳红¹(), 王连永², 杨强³, 李洪发¹, 李云洁¹

1.天津医科大学口腔医院正畸科,天津 300070
2.南开大学生命科学学院生物活性材料教育部重点实验室,天津 300071
3.天津医院脊柱外科,天津 300211

收稿日期:2017-05-05 修回日期:2017-09-02 出版日期:2018-02-07 发布日期:2018-02-01
作者简介:
滕彬宏,硕士,E-mail:tbhlianxi@qq.com
基金资助:
[基金项目] 国家自然科学基金（31300798,31470937,81572154）;天津市卫生局科技攻关重点项目（15KG125,16KG114）

Preparation and characterization of oriented scaffolds derived from cartilage extracellular matrix and silk fibroin

Binhong Teng¹(), Yanhong Zhao¹(), Lianyong Wang², Qiang Yang³, Hongfa Li¹, Yunjie Li¹

1. Dept. of Orthodontics, Stomato-logical Hospital of Tianjin Medical University, Tianjin 300070, China
2. The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China
3. Dept. of Spine Surgery, Tianjin Hospital, Tianjin 300211, China

Received:2017-05-05 Revised:2017-09-02 Online:2018-02-07 Published:2018-02-01
Supported by:
Supported by: The National Natural Science Foundation of China (31300798, 31470937, 81572154);The Key Project of Science and Technology Research of Tianjin Health Bureau (15KG125, 16KG114).

摘要/Abstract

摘要：

目的制备丝素蛋白（SF）-软骨脱细胞外基质（CECM）仿生支架材料,检测其理化性能特性。方法采用改良温度梯度热诱导相分离（TIPS）技术结合冷冻干燥法制备出CECM取向支架,然后将CECM浆料与制备好的SF溶液按照质量比1∶1混合后配制成质量分数6%的浆料,通过TIPS技术制备出SF-CECM复合取向支架。对SF-CECM复合取向支架进行扫描电子显微镜（SEM）观察和组织学染色,同时测定支架孔隙率、吸水性以及力学性能。结果 SEM观察可见,支架横断面呈多孔网状结构,纵剖面呈垂直的管状结构。组织学染色显示复合支架脱细胞彻底,有蛋白多糖、胶原成分,与天然软骨成分相似。支架孔隙率、吸水率和纵向压缩弹性模量分别为95.733%±1.010%、94.309%±1.302%和（65.40±4.09）kPa。结论 SF-CECM复合取向支架具有良好的理化特性和生物力学性能,有望成为较理想的组织工程软骨支架。

关键词: 软骨, 丝素蛋白, 细胞外基质, 取向支架, 组织工程

Abstract:

Objective This study aims to prepare oriented scaffolds derived from a cartilage extracellular matrix (CECM) and silk fibroin (SF) and use to investigate their physicochemical property in cartilage tissue engineering. Methods Oriented SF-CECM scaffolds were prepared from 6% mixed slurry (CECM：SF=1：1) through modified temperature gradient-guided thermal-induced phase separation, followed by freeze drying. The SF-CECM scaffolds were evaluated by scanning electron microscopy (SEM) and histological staining analyses and determination of porosity, water absorption, and compressive elastic modulus of the materials. Results The SEM image showed that the SF-CECM scaffolds contained homogeneous reticular porous structures in the cross-section and vertical tubular structures in the longitudinal sections. Histological staining showed that cells were completely removed, and the hybrid scaffolds retained proteogly can and collagen. The composition of the scaffold was similar to that of natural cartilage. The porosity, water absorption rate, and vertical compressive elastic modulus of the scaffolds were 95.733%±1.010%, 94.309%±1.302%, and (65.40±4.09) kPa, respectively. Conclusion The fabricated SF-CECM scaffolds exhibit satisfactory physicochemical and biomechanical properties and thus could be an ideal scaffold in cartilage tissue engineering.

Key words: cartilage, silk fibroin, extracellular ma-trix, oriented scaffolds, tissue engineering

中图分类号:

Q813.1

滕彬宏, 赵艳红, 王连永, 杨强, 李洪发, 李云洁. 丝素蛋白-软骨脱细胞外基质复合取向支架的制备及评价[J]. 华西口腔医学杂志, 2018, 36(1): 17-22.

Binhong Teng, Yanhong Zhao, Lianyong Wang, Qiang Yang, Hongfa Li, Yunjie Li. Preparation and characterization of oriented scaffolds derived from cartilage extracellular matrix and silk fibroin[J]. West China Journal of Stomatology, 2018, 36(1): 17-22.

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丝素蛋白-软骨脱细胞外基质复合取向支架的制备及评价

Preparation and characterization of oriented scaffolds derived from cartilage extracellular matrix and silk fibroin

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