下颌持续前导对成年大鼠髁突软骨骨形态发生蛋白表达及超微结构的影响

doi:10.7518/hxkq.2016.06.016

华西口腔医学杂志 ›› 2016, Vol. 34 ›› Issue (6): 632-638.doi: 10.7518/hxkq.2016.06.016

下颌持续前导对成年大鼠髁突软骨骨形态发生蛋白表达及超微结构的影响

杨帅¹,李雪²,高洁³,蔡毅志⁴,李荣慧⁴,王明国⁵

1.潍坊医学院口腔医学院，潍坊 261053；2.潍坊口腔医院口腔科，潍坊 261000；3.中国人民解放军第401医院口腔科，青岛 266071；4.山东大学口腔医学院，济南 250012；5.山东大学附属济南市中心医院口腔科，济南 250013

收稿日期:2016-01-15 修回日期:2016-07-10 出版日期:2016-12-01 发布日期:2016-12-01
通讯作者: 王明国，副主任医师，博士，E-mail：wmgsh@163.com
作者简介:杨帅，硕士，E-mail：907445117@qq.com
基金资助:
山东省科技发展项目（2014GSF11804）

Changes in ultrastructure and bone morphogenetic protein expression in reconstructed mandibular condylar cartilage under continuous mandibular advancement in adult rats

Yang Shuai¹, Li Xue², Gao Jie³, Cai Yizhi⁴, Li Ronghui⁴, Wang Mingguo⁵

1. School of Stomatology, Weifang Medical University, Weifang 261053, China; 2. Dept. of Stomatology, Weifang Oral Hospital, Weifang 261000, China; 3. Dept. of Stomatology, The 401st Hospital of PLA, Qingdao 266071, China; 4. School of Stomatology, Shandong University, Jinan 250012, China; 5. Dept. of Stomatology, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China) Supported by: The Science and Technology Development Program of Shandong Province (2014GSF11804). Correspondence: Wang Mingguo, E-mail: wmgsh@163.com.

Received:2016-01-15 Revised:2016-07-10 Online:2016-12-01 Published:2016-12-01

摘要/Abstract

摘要： 目的观察成年期大鼠在下颌持续前导作用下髁突软骨的改建以及超微结构的变化。方法将30只9周龄SD大鼠随机分为实验组和对照组，实验组佩戴上颌斜面导板，对照组不做任何处理，分别在第3、7、14、21、30天处死动物并取材，免疫组织化学染色检测骨形态发生蛋白2（BMP-2）在髁突中的表达及分布，透射电镜观察髁突软骨细胞的超微结构，Micro CT分析髁突骨质的变化。结果与对照组相比，实验组髁突中部和后部软骨增生明显，软骨细胞BMP-2阳性细胞率及灰度值在第7天开始增多，随时间延长而增强，髁突软骨细胞的超微结构出现细胞核固缩，核周微丝变少、脂滴变小、内质网腔隙肿胀、细胞外基质增宽变多等。Micro-CT显示实验组的新生骨和骨小梁厚度、骨小梁数量和分离度随时间延长而增加。结论在下颌持续前导下，成年期大鼠髁突软骨出现增生性改建，并存在BMP-2的高表达。

关键词: 髁, 软骨, 超微结构, 骨形态发生蛋白

Abstract: Objective This study investigated the reconstructed mandibular condylar cartilage and the ultrastructural variations in mandibular condylar cartilage in adult rats as a result of mandibular advancement. Methods Thirty 9-week-old male Sprague-Dawley rats were randomly divided into experimental and control groups. Rats in the experimental group were subjected to mandibular advancement. Rats were sacrificed on days 3, 7, 14, 21, 30. Sections were cut from condyles, and bone morphogenetic protein-2 (BMP-2) expression in condylar cartilage was examined through immunohistochemical analysis. Condylar cartilage samples were harvested, and ultrastructural changes in these samples were observed under Micro-CT and transmission electron microscope. Results Compared with the control group, the experimental group obviously displayed cartilage hyperplasia in the middle and rear of the condyle. Moreover, the number of BMP-2-positive cells in condylar cartilage and the gray value gradually increased in the experimental group on day 7 of the intervention. Ultrastructural changes, such as karyopyknosis, reduced microfilaments around the nucleus, reduction in size or even disappearance of lipid droplets, swelling of endoplasmic reticulum compartments, broadened and increased extracellular matrix, were observed in the condylar hypertrophic chondrocytes. Micro-CT revealed that the trabecula and the newly formed bone gradually thickened. Conclusion Hypertrophic remodeling of the condylar cartilage and high BMP-2 expression are observed in adult rats as a result of continuous mandibular advancement.

Key words: condyle, cartilage, ultrastructure, bone morphogenetic protein

中图分类号:

R 783.5

杨帅,李雪,高洁,蔡毅志,李荣慧,王明国. 下颌持续前导对成年大鼠髁突软骨骨形态发生蛋白表达及超微结构的影响[J]. 华西口腔医学杂志, 2016, 34(6): 632-638.

Yang Shuai, Li Xue, Gao Jie, Cai Yizhi, Li Ronghui, Wang Mingguo. Changes in ultrastructure and bone morphogenetic protein expression in reconstructed mandibular condylar cartilage under continuous mandibular advancement in adult rats[J]. West China Journal of Stomatology, 2016, 34(6): 632-638.

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下颌持续前导对成年大鼠髁突软骨骨形态发生蛋白表达及超微结构的影响

Changes in ultrastructure and bone morphogenetic protein expression in reconstructed mandibular condylar cartilage under continuous mandibular advancement in adult rats

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