华西口腔医学杂志 ›› 2016, Vol. 34 ›› Issue (6): 570-574.doi: 10.7518/hxkq.2016.06.004

• 基础研究 • 上一篇    下一篇

电纺聚己内酯引导骨再生膜的仿生矿化研究

邓霞1,白石2   

  1. 1.核工业四一六医院口腔科,成都 610051;2.重庆医科大学附属口腔医院种植科,重庆 400015
  • 收稿日期:2016-04-10 修回日期:2016-10-08 出版日期:2016-12-01 发布日期:2016-12-01
  • 通讯作者: 白石,主治医师,硕士,E-mail:2296059@qq.com
  • 作者简介:邓霞,副主任医师,博士, E-mail:xiad520@sina.com

Biomineralization of electrospun polycaprolactone-guided bone regeneration membrane

Deng Xia1, Bai Shi2   

  1. 1. Dept. of Stomatology, Nuclear of Industry 416 Hospital, Chengdu 610051, China; 2. Dept. of Implantology, College of Stomatology, Chongqing University of Medical Science, Chongqing 400015, China) Correspondence: Bai Shi, E-mail:2296059@qq.com.
  • Received:2016-04-10 Revised:2016-10-08 Online:2016-12-01 Published:2016-12-01

摘要: 目的 探讨组织工程化电纺聚己内酯支架材料的生物矿化特性及其用作引导骨组织再生膜的可能性。方法利用静电纺丝法制备聚己内酯(PCL)超细纤维膜支架,采用体外过饱和矿化液(SCS)浸泡法,在电纺膜上仿生沉积磷灰石。该复合材料采用扫描电子显微镜(SEM)、X射线衍射仪(XRD)、傅里叶变换红外光谱仪(FTIR)等方法进行结构表征;采用接触角实验评价其亲水性;将成骨细胞与复合材料共培养,用SEM观察并评价其细胞相容性。结果 PCL电纺薄膜由超细纤维交织形成三维多孔结构,随着矿化时间增加,PCL电纺纤维上沉积的结晶物数量增多,形态增大并覆盖整个薄膜表面,形成磷酸氢钙及类骨磷灰石晶体。成骨细胞在复合材料表面黏附、铺展,呈正常生长形貌。结论 PCL电纺膜经过SCS处理是一种简便有效的制备复合材料的仿生矿化方法,该材料具有良好的细胞相容性,在仿细胞外基质组织工程化材料及引导骨组织再生膜研制方面具有一定的应用前景。

关键词: 引导骨组织再生, 静电纺丝, 聚己内酯, 仿生矿化, 磷酸钙

Abstract: Objective To evaluate the biomineralization of the tissue-engineering electrospun polycaprolactone (PCL) scaffold and its potential use for guided bone regeneration (GBR) membranes. Methods PCL ultrafinefiber scaffolds were fabricated by electrospinning and then immersed in supersaturated calcification solution (SCS) for biomineralization investigation. The electrospun PCL scaffolds and the calcium phosphate coating were identified by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). Water-contact angles were also measured to evaluate the hydrophilicity of the modified surface. The biocompatibility of the composite was investigated by culturing osteoblasts on the scaffolds. Cell behavior was observed by SEM. Results The electrospun PCL scaffold was composed of ultrafine fibers and well-interconnected pores. The deposits on the fibers grew in number and size as the biomineralization time increased. Then, the covering of the whole PCL film was identified as dicalcium phosphate dehydrate and apatite. Good cell attachment and proliferation behavior were observed on the membranes. Conclusion The quick apatite formation on the surface of the PCL electrospun scaffold indicated that SCS biomineralization may be an effective approach for obtaining PCL/calcium phosphate composites. The cellular biocompatibility of the composite scaffold was preliminarily confirmed by the in vitro culture of osteoblasts on the scaffold. As such, the composite scaffold is a promising biomimetic extracellular matrix biomaterial for bone tissue engineering and GBR membranes.

Key words: guided bone regeneration, electrospun, polycaprolactone, biomineralization, calcium phosphate

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