华西口腔医学杂志 ›› 2016, Vol. 34 ›› Issue (3): 239-243.doi: 10.7518/hxkq.2016.03.005

• 基础研究 • 上一篇    下一篇

大鼠正畸牙移动过程中牙周膜骨硬化蛋白的表达

陈一文1,高尚1,许彤彤1,张佳慧1,李金城1,李金成1,张慧彦1,卢金金1,胡敏1,刘志辉2   

  1. 1.吉林大学口腔医院正畸科;2.修复科,长春130012
  • 收稿日期:2015-12-01 修回日期:2016-03-05 出版日期:2016-06-01 发布日期:2016-06-01
  • 通讯作者: 胡敏,教授,博士,E-mail:humin@jlu.edu.cn
  • 作者简介:陈一文,学士,E-mail:397329354@qq.com
  • 基金资助:
    国家自然科学基金(81470764);吉林省科技厅重点科技攻关科技计划(20140204066SF);大学生创新创业训练计划(2-013B78356)

Sclerostin expression in periodontal ligaments during movement of orthodontic teeth in rats

Chen Yiwen1, Gao Shang1, Xu Tongtong1, Zhang Jiahui1, Li Jincheng1, Li Jincheng1, Zhang Huiyan1, Lu Jinjin1, Hu Min1, Liu Zhihui2   

  1. 1. Dept. of Orthodontics, School of Stomatology, Jilin University, Changchun 130012, China; 2. Dept. of Prosthodontics, School of Stomatology, Jilin University, Changchun 130012, China) Supported by: The National Natural Science Foundation of China(81470764); Vital Science and Technology Project Supported by Jilin Provincial Science and Technology Department(20140204066SF); Undergraduate Training Programs for Innovation and Entrepreneurship(2013B78356). Correspondence: Hu Min, E-mail: humin@jlu.edu.cn.
  • Received:2015-12-01 Revised:2016-03-05 Online:2016-06-01 Published:2016-06-01

摘要: 目的 观察正畸牙移动过程中大鼠牙周组织中骨硬化蛋白(Sclerostin)的表达及分布,研究Sclerostin在正畸牙移动骨改建中的作用。方法 选取24只Wistar大鼠,安装加力装置,加载50 g力近中移动左侧第一磨牙,分别于安装加力装置后的0、1、3、5、7、14 d处死大鼠,用苏木精-伊红(HE)染色观察第一磨牙牙周组织形态学变化,抗酒石酸酸性磷酸酶(TRAP)染色观察破骨细胞的数量变化,免疫组织化学染色方法探究第一磨牙牙周膜中Sclerostin的表达变化。结果 HE染色显示随加力时间的延长压力侧骨组织破坏逐渐加重,免疫组织化学染色显示Sclerostin的表达逐渐增加,5 d时达到高峰,之后又逐渐降低,压力侧表达多于张力侧。结论 Sclerostin可能通过Wnt信号通路或者直接或间接控制骨形态发生蛋白参与了正畸牙移动骨改建过程。

关键词: 骨硬化蛋白, 牙周膜, 正畸牙移动

Abstract: Objective This study aims to observe the expression of Sclerostin during movement of orthodontic teeth and determine the effect of this protein on remodeling of periodontal tissues. Methods Twenty-four Wistar rats were chosen. Orthodontic forces were applied between the bilateral incisor and first molar to achieve mesial movement. Rats in each group were executed at different time points (0, 1, 3, 5, 7, 14 d). Morphology of periodontal tissue was observed by hematoxylineosin (HE) staining. The number of osteoclasts were observed by tartrate-resistant acid phosphatase (TRAP) staining. Sclerostin expression were observed by immunohistochemical staining. Results HE staining revealed that the resorption of alveolar bone intensified with prolonged movement. Results of immunohistochemical and TRAP staining revealed that Sclerostin expression and number of osteoclasts were related to duration of movement of orthodontic tooth. After staining for 5 days, the number of osteoclasts and Sclerostin expression reached their peak and then began to decline. The numbers of osteoclasts and the expression level of Sclerostin were higher at the compressive side than those at the tensive side. Conclusion Sclerostin affected orthodontic tooth movement by inhibiting the Wnt signaling pathway and by indirectly or directly controlling bone morphogenetic protein.

Key words: Sclerostin, periodontal ligament, orthodontic tooth movement

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