关键词: 变异链球菌, ffh基因, 荧光定量聚合酶链反应

Abstract:

Objective  This research aimed to detect the expression levels of ffh gene in Streptococcus mutans (S. mutans) UA159 under different pH conditions, analyze the effect of pH on the expression of ffh gene in S. mutans, and identify the factors regulating the ffh gene expression. Methods  Samples of S. mutans were collected at different growth stages (4 h, 18 h) and pH values (pH 4.0-7.0). Fluorescence quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the relative mRNA expression and trend of the target gene ffh in S. mutans at different growth stages and pH values. Results  qRT-PCR results showed that the ffh gene expression decreased along with pH at 4 h, but the expression increased with decreasing pH at 18 h. Under the same pH conditions, the ffh gene expression was significantly different between 4 h and 18 h (P<0.05). Conclusion  Growth stage and pH value influenced the ffh gene expression in S. mutans.

Key words: Streptococcus mutans,  , ffh gene,  , fluorescence quantitative real-time polymerase chain reaction