华西口腔医学杂志

• 专栏论著 • 上一篇    下一篇

全长型脾酪氨酸激酶在口腔鳞状细胞癌组织中的表达及其与肿瘤侵袭和转移的关系

王钊1,2  陈洁1,2  储伟明1,2  达明杰1,2  马露1,2  吴敏3  钟旖3  王子露1  宋晓萌1,2  吴煜农1,2   

  1. 1.南京医科大学口腔疾病研究江苏省重点实验室;2.南京医科大学附属口腔医院口腔颌面外科;3.口腔病理科,南京 210029
  • 收稿日期:2015-03-12 修回日期:2015-07-05 出版日期:2015-10-01 发布日期:2015-10-01
  • 通讯作者: 吴煜农,主任医师,博士,E-mail:wynoms@gmail.com
  • 作者简介:王钊,住院医师,学士,E-mail:wangzhao8178@163.com
  • 基金资助:

    上海市口腔医学重点实验室开放基金科研项目(2012/K-F02)

Expression of full-length spleen tyrosine kinase in the oral squamous cell carcinoma and its relationship with tumor invasion and metastasis

Wang Zhao1,2, Chen Jie1,2, Chu Weiming1,2, Da Mingjie1,2, Ma Lu1,2, Wu Min3, Zhong Yi3, Wang Zilu1, Song Xiaomeng1,2, Wu Yunong1,2.   

  1. 1. Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing 210029, China; 2. Dept. of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, China; 3. Dept. of Pathology, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, China
  • Received:2015-03-12 Revised:2015-07-05 Online:2015-10-01 Published:2015-10-01

摘要:

目的  通过检测口腔鳞状细胞癌(OSCC)组织中全长型脾酪氨酸激酶[SYK(L)]的表达、与淋巴结转移的相关性分析,探讨SYK(L)对OSCC发生发展和转移的影响。方法  采用实时荧光定量聚合酶链反应(RT-qPCR)、免疫印迹(Western blot)、免疫组织化学法检测SYK(L)在27例OSCC及其癌旁组织中表达的差异,并采用14例正常口腔黏膜作为对照,探讨其与OSCC发生及转移的关系。结果  OSCC组织中SYK(L)表达量明显低于正常牙龈组织(P<0.01),同一患者癌组织中SYK(L)表达量比癌旁组织低(P<0.05);淋巴结转移组患者的SYK(L)表达量普遍低于未转移组患者(P<0.05)。结论  SYK(L)可能与OSCC的发生和发展有关,作为抑癌基因在肿瘤的淋巴结转移过程中起至关重要的作用。

关键词: 口腔鳞状细胞癌, 全长型脾酪氨酸激酶, 淋巴结转移, 选择性拼接

Abstract:

Objective  To investigate the expression of full-length spleen tyrosine kinase [SYK (L)] mRNA and protein in human oral squamous cell carcinoma (OSCC) as well as its possible effects on the invasion and metastasis of OSCC. Methods  The expression of SYK (L) was detected in 27 cases of OSCC tissues and its matched adjacent non-cancerous tissues by real-time quantitative polymerase chain reaction (RT-qPCR), Western blot, and immunohistochemistry. Fourteen cases of normal oral gingival tissues were also analyzed as a normal control. Results  Reduced mRNA and protein expression of SYK (L) in OSCC tissues was observed compared with that in normal oral gingival tissues (P<0.01) and adjacent non-cancerous tissues (P<0.05). SYK(L) expression was significantly associated with lymph-node metastasis (P<0.05). Conclusion  SYK(L) is a candidate tumor suppressor for OSCC tissues, and has an inhibitive effect on the initiation, proliferation, and lymph-node metastasis of human OSCC.

Key words: oral squamous cell carcinoma, full-length spleen tyrosine kinase, lymph node metastasis, alternative-splice