华西口腔医学杂志

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4种纳米羟磷灰石复合材料对粪肠球菌抗菌性能的初步研究

刘奕1 周荣静2 吴红崑3   

  1. 1.四川省医学科学院•四川省人民医院口腔科,成都 610072;2.聊城市人民医院口腔科,聊城 252000;3.口腔疾病研究国家重点实验室 华西口腔医院特需门诊(四川大学),成都 610041
  • 出版日期:2015-06-01 发布日期:2015-06-01
  • 通讯作者: 吴红崑,教授,博士,E-mail:whk-mo@263.net
  • 作者简介:刘奕,主治医师,博士,E-mail:liuyi82404@hotmail.com
  • 基金资助:

    四川省科技应用基础计划基金资助项目(2008JY0037)

Study on the antibacterial activity of four kinds of nano-hydroxyapatite composites against Enterococcus faecalis

Liu Yi1, Zhou Rongjing2, Wu Hongkun3.   

  1. 1. Dept. of Oral Medicine, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, Chengdu 610072, China; 2. Dept. of Oral Medicine, Liaocheng People’s Hospital, Liaocheng 252000, China; 3. State Key Laboratory of Oral Diseases, Dept. of Special Dental Care, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Online:2015-06-01 Published:2015-06-01

摘要:

目的 筛选一种对粪肠球菌有抑菌效能的纳米羟磷灰石复合材料。方法 选用载银纳米羟磷灰石(Ag/ nHA)、载钇纳米羟磷灰石(Yi/nHA)、载铈纳米羟磷灰石(Ce/nHA)、普通纳米羟磷灰石(nHA)为实验材料,分别采用琼脂扩散法和琼脂稀释法,通过计量不同实验材料对粪肠球菌的抑菌环直径和最小抑菌浓度(MIC),评价4种纳米复合材料对粪肠球菌的影响。结果 琼脂扩散法显示Ag/nHA周围出现明显的抑菌环,而Yi/nHA、Ce/ nHA、nHA周围均无抑菌环。琼脂稀释法显示Ag/nHA对粪肠球菌的MIC为1.0 g·L-1;其他材料即使浓度高达32.0 g·L-1也未表现出抑制粪肠球菌的作用。结论 Ag/nHA对浮游状态的粪肠球菌有较好的抑制作用,而Yi/nHA、Ce/nHA、nHA在实验浓度范围内均无明显地抑制粪肠球菌的作用。

关键词: 纳米羟磷灰石, 粪肠球菌, 抑菌性能

Abstract:

Objective  This study aims to compare and determine a kind of nano-hydroxyapatite composite material with good antibacterial efficacy on Enterococcus faecalis (E. faecalis) in vitro. Methods  We investigated the antimicrobial activity of four kinds of nano-hydroxyapatite composites, namely, silver/hydroxyapatite composite nanoparticles (Ag/nHA), yttrium/hydroxyapatite composite nanoparticles (Yi/nHA), cerium/hydroxyapatite composite nanoparticles (Ce/nHA), and hydroxyapatite nanoparticles (nHA), against E. faecalis in vitro using the agar diffusion and broth dilution method by measuring the growth inhibition zone and the minimum inhibitory concentration (MIC), respectively. Results  The agar diffusion test results showed that Ag/nHA displayed an obvious growth inhibition zone, whereas Yi/nHA, Ce/nHA, and nHA showed no influence on E. faecalis. The MIC value of Ag/nHA was 1.0 g·L-1, and the three other materials had no effect on E. faecalis even at the high concentration of 32.0 g·L-1. Conclusion  Ag/nHA display a potential antimicrobial efficacy to planktonic E. faecalis. Whereas, the three other kinds of nano-hydroxyapatite composites (Yi/nHA, Ce/nHA, nHA) show no influence.

Key words: nano-hydroxyapatite, Enterococcus faecalis, antibacterial activity