华西口腔医学杂志

• 基础研究 • 上一篇    下一篇

海藻酸钙-纳米羟磷灰石骨修复材料的制备及部分生物相容性测定

王彦梅1  何家才1,2  李全利1,2  沈际佳3   

  1. 1.安徽医科大学附属口腔医院颌面外科;2.安徽省口腔临床医学重点学科,安徽省口腔疾病研究省级实验室,中央与地方共建口腔医学中心实验室;
    3.安徽医科大学病原生物学教研室,合肥 230032
  • 出版日期:2014-02-01 发布日期:2014-02-01
  • 通讯作者: 何家才,主任医师,博士,E-mail:hejiacai@163.com
  • 作者简介:王彦梅,硕士, E-mail:WangYanmei2008_good@126.com

Preparation of sodium alginate-nanohydroxyapatite composite material for bone repair and its biocompatibility 

 Wang Yanmei1, He Jiacai1,2, Li Quanli1,2, Shen Jijia3.   

  1. 1. Dept. of Oral and Maxillofacial Surgery, Stomatological Hospital Affiliated to Anhui Medical University, Hefei 230032, China; 2. Key Discipline of Clinical Science of Stomatology in Anhui Province, Province Laboratory of Oral Diseases, Central and Local Working Together for the Oral Medical Center Labora-tory, Hefei 230032, China; 3. Dept. of Microbiology and Parasitology, Anhui Medical University, Hefei 230032, China
  • Online:2014-02-01 Published:2014-02-01

摘要:

 目的  制备海藻酸钙-纳米羟磷灰石复合材料,探讨其作为骨修复材料的可行性。方法  应用化学交联及冷冻干燥技术制备海藻酸钙 -纳米羟磷灰石复合材料,通过扫描电镜( SEM)和 X光衍射( XRD)对材料性能进行表征,液体置换法测定孔隙率。将第 5代骨髓基质干细胞( BMSCs)接种至材料上,倒置显微镜及 SEM观察细胞生长情况。材料浸提液与 BMSCs共培养,甲基噻唑基四唑( MTT)法检测接种后 1、3、5d的相对增殖率( RGR),并评估毒性分级。新鲜犬血加入材料浸提液进行溶血试验,分光光度计测定光密度值并计算溶血率。结果  制备的海藻酸钙 -纳米羟磷灰石复合材料具有多孔性,孔隙率达( 88.6±4.5)%。倒置显微镜及 SEM观察可见,细胞在材料周围及表面伸展充分、生长旺盛。材料浸提液培养的细胞具有较好的活力,其毒性分级为 1级。溶血试验结果表明复合材料的溶血率为 1.28%,满足医用生物材料的要求。结论  本研究制备的复合材料具有较高的孔隙率和良好的生物相容性。

关键词:  , 海藻酸钙, 纳米羟磷灰石, 复合材料, 骨髓基质干细胞, 生物相容性

Abstract:

Objective To prepare sodium alginate-nanohydroxyapatite composite material and to explore its feasibility as a bone repair material. Methods  Sodium alginate-nanohydroxyapatite composite material was prepared using chemical cross-linking and freeze-drying technology. The composite was characterized by X-ray diffraction (XRD) and scanning electron microscope(SEM)and its porosity wasmeasured byliquiddisplacement method.The fifth passage of bonemarrow stromal stem cells (BMSCs) were incubated on the composite material and then growth was observed by inverted micros-cope and SEM. BMSCs were cultured with liquid extracts of the material, methyl thiazolyl tetrazolium (MTT) assay was used to calculate the relative growth rate (RGR) on 1, 3, 5 d and to evaluate the cytotoxicity. Fresh dog blood was added into the liquidextractstoconducthemolysistest,thespectrophotometerwasusedtodeterminetheopticaldensity(OD)andtocalculate the hemolysis rate. Results Sodium alginate-nanohydroxyapatite composite material displayed porosity, the porous pore rate was (88.6±4.5)%. BMSCs showed full stretching and vigorous growth under inverted microscope and SEM. BMSCs cultured with liquid extracts of the material had good activities. The toxicity of composite material was graded as 1. Hemolysis test results showed that the hemolysis rate of the composite material was 1.28%, thus meeting the requirement of medical biomaterials. Conclusion  The composite material fabricated in
thisstudyhashighporosityandgoodbiocompatibility.

Key words: sodium alginate, nanohydroxyapatite, compositematerial, bonemarrowstromalstemcells, biocompatibility