华西口腔医学杂志

• 专栏论著 • 上一篇    下一篇

雌激素对人胚髁突软骨细胞增殖分化的影响

庞磊1,2 张跃蓉1 刘忠林1   

  1. 1.遵义医学院口腔学院口腔解剖生理学教研室，遵义 563003；2.湖北医药学院人体解剖教研室，十堰 442000
  • 出版日期:2013-12-01 发布日期:2013-12-01
  • 通讯作者: 张跃蓉，Tel：0852-8609757
  • 作者简介:庞磊（1983—），女，湖北人，主治医师，硕士
  • 基金资助:

    贵州省科学技术基金资助项目[黔科合J字（2010）2170号]

Effects of estrogen on the proliferation and differentiation of human embryo mandibular condylar chondrocytes

Pang Lei1,2, Zhang Yuerong1, Liu Zhonglin1.   

  1. 1. Dept. of Oral Anatomy and Physiology, College of Stomatology, Zunyi Medical College, Zunyi 563003, China; 2. Dept. of Human Anatomy, Hubei University of Medical, Shiyan 442000, China
  • Online:2013-12-01 Published:2013-12-01

RichHTML

0

PDF (PC)

730

被引次数

10

摘要：

目的 观察不同浓度的雌激素作用于体外培养的人胚髁突软骨细胞（MCCs），探讨雌激素对MCCs增殖分化的影响。方法 体外培养MCCs，甲苯胺蓝及Ⅱ型胶原免疫细胞化学染色法鉴定；甲基噻唑基四唑（MTT）法检测不同浓度雌激素对MCCs增殖与分化的影响。结果 高浓度（10-6 mol·L-1）、低浓度（10-12 mol·L-1）雌二醇抑制MCCs增殖分化，生理浓度（10-10、10-8 mol·L-1）雌二醇促进MCCs增殖分化，且随作用时间延长，促进或抑制作用增强。结论 雌激素对体外培养的人胚MCCs增殖分化具有双向调节作用，呈时间和剂量依赖性。表明雌激素对维持颞下颌关节的正常功能具有重要意义，可能在颞下颌关节病的发生和进程中发挥作用。

关键词: 髁突软骨细胞, 雌激素, 增殖, 人胚

Abstract:

Objective  To determine the effects of different estrogen concentrations on the proliferation and differentiation of human embryo mandibular condylar chondrocytes (MCCs). Methods  In vitro-cultured MCCs were incubated and treated with estrogen at different concentrations. The MCCs were identified by toluidine blue and type Ⅱ collagen immunocytoche-mical methods, and cell proliferation was monitored using methyl thiazolyl tetrazolium assay. Results  The addition of 10-12 or 10-6 mol·L-1 exogenous estrogen inhibited the growth of MCCs, whereas addition of 10-10 or 10-8 mol·L-1 exogenous estrogen promoted growth. These estrogen effects were time-dependent. Conclusion  Estrogen can affect cellular MCCs proliferation and differentiation in a biphasic manner depending on the concentration. Estrogen may be vital in the maintenance of normal functions of the temporomandibular joint (TMJ) and may be involved in the progression of certain TMJ diseases.

Key words: mandibular condylar chondrocytes, estrogen, proliferation, human embryo

蜀ICP备09013973号-2
地址：成都市人民南路三段14号　电话：(028)85503479　传真：(028)85503479　Email：hxkqyxzz@vip.163.com
本系统由北京玛格泰克科技发展有限公司设计开发 技术支持：support@magtech.com.cn