关键词: 牙科金属, 浸提液, 成纤维细胞L929, 凋亡, 免疫组织化学

Abstract:

Objective   To investigate the effects of the leaching liquids of 4 differents kinds of dental alloys(Au alloy, Ag-Pd alloy, Co-Cr alloy, Ni-Cr alloy) on apoptosis related gene and protein of fibroblast L929. Methods     The L929 cells of mouse were treated in vitro with leaching liquids of 4 different kinds of dental alloys, Au alloy(group A), Ag-Pd alloy(group B), Co-Cr alloy(group C) and Ni-Cr alloy(group D). The RPMI1640 cell medium containing 10% fetal calf serum was served as a control(group E). The effects of these alloys on the expression of caspase-3, 8, 9 of L929 cells were examined by reverse transcription-polymerase chain reaction(RT-PCR) and immunohistochemistry method. Results   After 48 h culture, the mRNA levels of caspase-3 and caspase-9 demonstrated sig-nificant differences between the groups expect group A and group E. The mRNA levels of caspase-8 had no change in all groups. The expression of caspase-3 and caspase-9 were significant differences between the groups expect group A and C, group B and D. The expression of caspase-8 had no change in all groups. Conclusion   The leaching liquids of 4 different kinds of dental alloys expect Au alloy may induce cell apoptosis through mitochondrion pathway.

Key words: dental alloy, leaching liquid, fibroblast L929, apoptosis, immunohistochemistry