华西口腔医学杂志

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人β防御素-4在牙龈组织表达的新发现

李欣忆1  段丁瑜1  王盼盼1  韩波2  徐屹1   

  1. 1.口腔疾病研究国家重点实验室,华西口腔医院牙周科,四川大学; 2.口腔颌面外科门诊,成都610041
  • 出版日期:2013-04-01 发布日期:2013-04-01
  • 通讯作者: 徐屹,Tel:028-85502343
  • 作者简介:李欣忆(1988—),女,重庆人,硕士
  • 基金资助:

    国家自然科学基金资助项目(30872872);四川省科技厅科技支撑计划基金资助项目(2011FZ0044)

New finding of the expression of human β defensin-4 in healthy gingiva

Li Xinyi1, Duan Dingyu1, Wang Panpan1, Han Bo2, Xu Yi1.   

  1. 1. State Key Laboratory of Oral Diseases, Dept. of Periodontology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; 2. State Key Laboratory of Oral Diseases, Dept. of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Online:2013-04-01 Published:2013-04-01

摘要:

目的  探讨牙龈组织中是否存在β防御素-4(hBD-4)及其分布情况。方法    收集健康牙龈标本。采用免疫组织化学法检测18例牙龈组织标本hBD-4蛋白水平的分布;提取组织RNA,进行实时荧光定量逆转录聚合酶链反应(real time RT-PCR)检测30例牙龈标本中hBD-4基因的表达水平。结果  免疫组织化学检测结果显示,18例牙龈样本中有13例检出hBD-4;real time RT-PCR检测结果显示,30例牙龈组织中4例样本检测出hBD-4表达。结论    hBD-4在健康牙龈组织中有不同水平的表达和分布,可能在维持牙周组织健康中起作用。

关键词: 牙龈, 人β防御素-4, 免疫组织化学, 实时荧光定量逆转录聚合酶链反应

Abstract:

Objective  To investigate the expression and the distribution of human β defensin (hBD)-4 in healthy gingiva. Methods  Healthy gingival specimens were collected. The expression of hBD-4 peptides in 18 gingival specimens were detected by immunohistochemistry. The hBD-4 mRNA were determined in freshly isolated gingival tissue by real time reverse transcription-polymerase chain reaction (real time RT-PCR) in 30 gingival specimens. Results  In 18 gingival specimens, hBD-4 peptides were expressed in 13 gingival specimens. In 30 gingival specimens, hBD-4 were detected in 4 gingival specimens by real time RT-PCR. Conclusion  The distribution and the expression levels of hBD-4 are different in healthy gingiva. This result may suggest that the hBD-4 play a role in maintaining the periodontal health.

Key words: gingiva, human &beta, defensin -4, immunohistochemistry, real time reverse transcription-polymerase chain reaction