华西口腔医学杂志

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改性后的骨形态发生蛋白-2聚乳酸纳米微球缓释系统促进下颌骨缺损修复的研究

罗菲1  卢来春2  曾勇1  赵智亮1  祝金香1  张纲1   

  1. 1.第三军医大学新桥医院口腔科,重庆 400037;2.第三军医大学大坪医院药剂科,重庆400038
  • 出版日期:2013-04-01 发布日期:2013-04-01
  • 通讯作者: 张纲,Tel:023-68774645
  • 作者简介:罗菲(1987—),女,浙江人,硕士
  • 基金资助:

    重庆市自然科学基金资助项目(CSTC2009BB5334);国家自然科学基金资助项目(8127109)

The effect of modified bone morphogenetic protein-2 polylactic acid nanospheres sustained-release system on promoting mandibular defect repair

Luo Fei1, Lu Laichun2, Zeng Yong1, Zhao Zhiliang1, Zhu Jinxiang1, Zhang Gang1   

  1. 1. Dept. of Stomatology, Xinqiaa Hospital, The Third Military Medical University, Chongqing 400037, China; 2. Dept. of Pharmacy, Daping Hospital, The Third Military Medical University, Chongqing 400038, China
  • Online:2013-04-01 Published:2013-04-01

摘要:

目的  探讨改性后聚乳酸(PLA)包裹骨形态发生蛋白-2(BMP-2)制备的纳米微球缓释系统对兔下颌骨缺损的修复效果。方法    将PLA进行接枝聚合反应改性后,应用超声乳化法制备PLA纳米微球(PLA-N)、BMP-2-PLA 纳米微球(BMP-2-PLA-Ns)凝胶。将45只家兔随机分为3组:空白组、PLA-Ns凝胶组(对照组)、BMP-2-PLA-Ns凝胶组(实验组),建立骨缺损动物模型,对照组植入PLA-Ns凝胶,实验组植入BMP-2-PLA-Ns凝胶,空白组不予特殊处理。术后第1、2、4周处死家兔,截取缺损区颌骨段,进行影像学、苏木精-伊红(HE)染色、PCNA免疫组织化学染色观察。结果  影像学观察可见:实验组骨缺损区修复良好,阴影不明显,修复效果好于对照组和空白组。HE染色观察可见:实验组和对照组有大量新生血管和继发性骨痂形成,实验组骨痂比例明显高于对照组和空白组。免疫组织化学观察可见:第1、2周,实验组PCNA阳性软骨细胞多于对照组和空白组;第4周,各组PCNA阳性细胞均罕见,PCNA阳性细胞检出率低于第1、2周。结论    BMP-2-PLA-NS缓释系统能明显促进下颌骨缺损修复。

关键词: 聚乳酸, 骨形态发生蛋白, 下颌骨, 骨缺损, 修复

Abstract:

Objective  To investigate the effect of modified bone morphogenetic protein -2 polylactic acid nanospheres (BMP-2-PLA-Ns) sustained-release system on rabbit mandibular defect repair. Methods  The polylactic acid snanospheres (PLA-Ns) and BMP-2-PLA -Ns were prepared by ultrasonic emulsification after graft polymerization. Forty-five rabbits were randomly divided into 3 groups: Blank group, PLA-Ns gel group (control group), and BMP- 2-PLA-Ns gel group (experimental group). The rabbit mandibular defect models were established. The defect area of control group was implanted with PLA-Ns gel, meanwhile, the experimental group was implanted with BMP-2-PLA-Ns gel, the blank group experienced no special handling. Rabbits were killed in 1, 2, 4 weeks after operation and the iconography, hematine eosin (HE) staining and PCNA immunohistochemistry were used to detect the reparative effect on rabbit mandible defects. Results  Image observation showed that bone defect repair in the experimental group was well and the shadow was not obvious. Better repair effect was seen compared with the control group and blank group. HE staining showed that the experimental group and the control group had a large number of neovascularization and secondary callus formation, callus in experimental group was obviously higher than that of control group and blank group. Immunohistochemical observation showed that the experimental group' s PCNA positive chondrocytes were more than those in the control group and the blank group in the first 2 weeks; all groups of PCNA positive cells were rare in the fourth week, PCNA positive expression rate of the fourth week was lower than that of the first 2 weeks. Conclusion  The modified BMP-2-PLA-Ns sustained-release system promotes mandibular defect repair obviously.

Key words: polylactic acid, bone morphogenetic protein, mandible, bone defect, repair