华西口腔医学杂志 ›› 2012, Vol. 30 ›› Issue (5): 487-492.doi: 10.3969/j.issn.1000-1182.2012.05.009

• 临床研究 • 上一篇    下一篇

低强度脉冲超声波联合引导骨组织再生促进牙周骨缺损修复的实验研究

蒋欣益1 杨霁2 柴召午3 宋锦璘1 邓锋1 王智彪4   

  1. 1.重庆医科大学附属口腔医院正畸科; 重庆市口腔疾病与生物医学研究中心, 重庆401147;2.重庆市铜梁县人民医院口腔科, 重庆402560; 3.重庆三峡中心医院口腔科, 重庆404000;4.重庆市超声医学工程重点实验室省部共建国家重点实验室培育基地, 重庆400016
  • 收稿日期:2011-12-12 修回日期:2012-02-20 出版日期:2012-10-01 发布日期:2012-10-01
  • 通讯作者: 宋锦璘,Tel:023-88860108
  • 作者简介:蒋欣益(1987—),女,四川人,硕士
  • 基金资助:

    国家自然科学基金资助项目(30870754);重庆市发展和改革委员会高技术产业重大产业技术开发基金资助项目[渝发改技(2007)1110号];重庆市科委自然科学基金资助项目(CSTC2010BB-5355)

Low intensity pulsed ultrasound irradiating combined with guided bone regeneration for promoting the repair effect of periodontal bone defect

Jiang Xinyi1, Yang Ji2, Chai Zhaowu3, Song Jinlin1, Deng Feng1, Wang Zhibiao4.   

  1. 1. Dept. of Orthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University; Chongqing Research Center for Oral Diseases and Biomedical Science, Chongqing 401147, China; 2. Dept. of Stomatology, Tongliang County People’s Hospital of Chongqing, Chongqing 402560, China; 3. Dept. of Stomatology, Chongqing Three Gorges Central Hospital, Chongqing 404000, China; 4. Chongqing Key Laboratory of Ultrasound Medical Engineering Cofounded
    by Chongqing and Ministry of Science and Technology, Chongqing 400016, China)
  • Received:2011-12-12 Revised:2012-02-20 Online:2012-10-01 Published:2012-10-01

摘要:

目的探讨低强度脉冲超声波(LIPUS)联合引导骨组织再生(GBR)对Beagle犬尖牙牙周骨缺损的修复效应。方法将8只Beagle犬中每只犬的4颗尖牙随机分配到4组:实验1组(LIPUS处理+GBR+自体骨移植组),实验2组(LIPUS处理+自体骨移植组),实验3组(GBR+自体骨移植组),空白对照组。在犬尖牙颊侧区根中1/3处制备牙周骨缺损模型,根据分组,自体骨移植后,实验1组和2组采用LIPUS处理20 min·d-1,超声强度为30 mW·cm-2,实验1、3组植入Bio-Gide胶原膜。在LIPUS处理6、8周后处死Beagle犬,对各组尖牙牙周骨缺损区进行Micro-CT检测和分析。结果临床观察各组牙周骨缺损面积均不同程度缩小。Micro-CT检测分析表明,各组6周与8周时的骨小梁数目、骨小梁厚度和骨小梁体积均无统计学差异(P>0.05),而各组之间的骨组织测量指标均有统计学差异(P<0.05),实验1组新生骨量最多。结论LIPUS具有促进牙周骨缺损修复的潜能,LIPUS与GBR结合可能更有利于牙周骨缺损的修复与再生。

关键词: 低强度脉冲超声波, 引导骨组织再生, 骨缺损, 修复效应

Abstract:

Objective To study the repair effect of low intensity pulsed ultrasound(LIPUS) irradiating combined with guided bone regeneration(GBR) on the defect of Beagle dog canines periodontal bone. Methods Four canine teeth of every beagle dog(8 beagle dogs) were randomly distributed: Group 1(LIPUS disposal + GBR+ autogenous bone graft group), group 2(LIPUS disposal + autogenous bone graft group), group 3(GBR + autogenous bone graft group), blank control group. The model of periodontal bone defect was established in the 1/3 part of the root buccal area. According to the group division, autogenous bone were grafted, group 1 and group 2 were disposed by LIPUS 20 min·d-1. The intensity of ultrasound were 30 mW·cm-2. Group 1 and group 3 were injected with Bio-Gide collagen membrane. The beagle dogs were executed at 6 and 8 weeks of the disposal of LIPUS and then Micro-CT test and analysis were conducted to periodontal bone defect area of each group. Results By clinical observation, there were different degrees of shrinkages of the area of periodontal bone defect in each group. The Micro-CT test analysis indicated that there was a statistical difference among the number, the thickness as well as the size of bone trabecula of each group
(P <0.05). However, there was no statistical difference (P >0.05) in terms of bone issue measurement index between 6 to 8 weeks of each group. Group 1 had the most new bone. Conclusion LIPUS has the potential to promote the repair of periodontal bone defect. Therefore the combination of LIPUS and GBR may be more conducive to the repair and regeneration of periodontal bone defect.

Key words: low intensity pulsed ultrasound, guided bone regeneration, bone defect, repair effect