关键词: 舌鳞状细胞癌, 肿瘤干细胞, 醛脱氢酶, 基因

Abstract:

Objective To screen the differentially expressed genes of stem-like cells in tongue squamous cell carcinoma using suppression subtractive hybridization（SSH）. Methods The cells with high and low expression of aldehyde dehydrogenase（ALDHbr and ALDHlow） were isolated respectively from human tongue squamous cell carcinoma Tca8113 cell line. SSH was used to identify differentially expressed genes in those two groups. Positive cell clones with high/low-expressed ALDH were screened randomly for the genetic sequences and function analysis by the bioinformatics technology. Results Total 62 differentially expressed genes were identified, most of which got involved in regulation of cell cycle, cellular differentiation and other biology activities as gene ontology（GO） analysis indicated. Signal pathway analysis revealed 12 pathways related to 11 genes changed significantly. Conclusion Several potential novel biomarker candidates of tongue cancer stem cells were identified and they may provide important insights into the functions and characteristics of human tumor stem cell.

Key words: tongue squamous cell carcinoma, tumor stem cell, aldehyde dehydrogenase, gene