华西口腔医学杂志 ›› 2012, Vol. 30 ›› Issue (4): 346-349.doi: 10.3969/j.issn.1000-1182.2012.04.004

• 基础研究 • 上一篇    下一篇

荷瘤鼠及口腔癌患者热化疗前后T淋巴细胞亚群、白细胞介素-2和肿瘤坏死因子-α水平的变化

史春生1 金慧军2 赵君3 王升志2 毛祖彝3   

  1. 1.烟台市福山区人民医院口腔科, 烟台265500;2.青岛大学医学院附属烟台毓璜顶医院口腔颌面外科, 烟台264000;3.口腔疾病研究国家重点实验室, 四川大学, 成都610041  
  • 收稿日期:2011-08-05 修回日期:2012-03-06 出版日期:2012-08-01 发布日期:2012-08-01
  • 通讯作者: 王升志,Tel:0535-6691999-81603
  • 作者简介:史春生(1965—),男,山东人,副主任医师,学士
  • 基金资助:

    山东省优秀中青年科学家奖励基金资助项目(03BS038)

Change of T lymphocyte subsets, interleukin-2 and tumor necrosis factor-α in tumor- bearing mice and patients with oral cancer receiving thermo-chemotherapy

Shi Chunsheng1, Jin Huijun2, Zhao Jun3, Wang Shengzhi2, Mao Zuyi3.   

  1. 1. Dept. of Stomatology, People’s Hospital of Fushan District of Yantai City, Yantai 265500, China; 2. Dept. of Oral and Maxillofacial Surgery, Yantai Yuhuangding Hospital, Qingdao University Medical College, Yantai 264000, China; 3. State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China
  • Received:2011-08-05 Revised:2012-03-06 Online:2012-08-01 Published:2012-08-01

摘要:

目的探讨荷瘤鼠及口腔癌患者热化疗前后T淋巴细胞亚群、白细胞介素(IL)-2和肿瘤坏死因子(TNF)-α活性水平的变化,并初步探讨三者之间的相关性。方法经热化疗处理后,采用甲基噻唑基四唑(MTT)法检测荷瘤鼠淋巴细胞转化指数(LTI)及IL-2、TNF-α水平,MTT法检测口腔癌患者IL-2、TNF-α水平,并采用3H-胸腺嘧啶核苷(3H-TdR)掺入法行淋巴细胞转化实验及CD4+、CD8+ T细胞亚群检测。结果高温联合平阳霉素治疗组(HP组)荷瘤鼠LTI、IL-2和TNF-α活性与正常对照组(N组)荷瘤鼠差异无统计学意义(P>0.05),而明显高于平阳霉素治疗组(P组)及不治疗组(NT组)(P<0.01)。临床实验中热化疗后口腔癌患者LTI、CD4+细胞数目及CD4+/CD8+比值明显提高,IL-2和TNF-α活性水平明显升高(P<0.01)。结论荷瘤宿主的LTI及IL-2和TNF-α活性水平在热化疗后明显升高,IL-2和T细胞之间有明显的相关性。

关键词: 热化疗, T淋巴细胞亚群, 白细胞介素-2, 肿瘤坏死因子-&alpha

Abstract:

Objective To investigate the change of T lymphocyte subsets, interleukin(IL)-2 and tumor necrosis factor(TNF)-α in the tumor- bearing mice and patients with oral cancer receiving thermo-chemotherapy, and investigate the correlation among them. Methods After treatments, the expression of lymphocyte transformation index(LTI), IL-2 and TNF-α in the tumor-bearing mice were detected with methyl thiazolyl tetrazolium(MTT), the expression of IL-2 and TNF-α in the patients with oral cancer were detected with MTT, the expression of LTI, CD4+ and CD8+ were detected with 3H-TdR incorporation. Results LTI, IL-2 and TNF-α of thermo-chemotherapy group(HP group) had no significant difference comparing with those of normal mice group(N group)(P>0.05), but which were significantly
higher than those of chemotherapy group(P group) and no treatment group(NT group)(P<0.01). In clinical trials, the expression of LTI, CD4+, CD4+/CD8+, IL-2 and TNF-α on oral cancer patients after thermo-chemotherapy were significantly higher than those before thermo-chemotherapy(P<0.01). Conclusion After thermo-chemotherapy, the expression of LTI, IL-2 and TNF-α of tumor-bearing hosts are significantly improved, there is a significant correlation between IL-2 and T cell.

Key words: thermo-chemotherapy; T lymphocyte subsets; interleukin-2; tumor necrosis factor-&alpha