华西口腔医学杂志

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吸烟和细胞色素P450 1A1-MspⅠ、谷胱甘肽硫转移酶T1基因多态性与口腔癌的相关性研究

郭李柯1 张超贤2 史淑敏1 郭晓凤3   

  1. 1.新乡医学院第一附属医院口腔科; 2.消化内科, 卫辉453100;3.杭州市第六人民医院肝病科, 杭州310014
  • 收稿日期:2012-04-25 修回日期:2012-04-25 出版日期:2012-04-01 发布日期:2012-04-01
  • 通讯作者: 张超贤,Tel:13598738146
  • 作者简介:郭李柯(1975—),女,河南人,护师,学士

Correlation between smoking and the polymorphisms of cytochrome P450 1A1-MspⅠand glutathione S-transferase T1 genes and oral cancer

Guo Like1, Zhang Chaoxian2, Shi Shumin1, Guo Xiaofeng3   

  1. 1. Dept. of Stomatology, The First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, China; 2. Dept. of Gastroenterology, The First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, China; 3. Dept. of Hepatology, The Sixth People’s Hospital of Hangzhou, Hangzhou 310014, China
  • Received:2012-04-25 Revised:2012-04-25 Online:2012-04-01 Published:2012-04-01
  • Contact: Zhang Chaoxian,Tel:13598738146
  • About author:Guo Like(1975—),女,河南人,护师,学士

摘要:

目的探讨吸烟和细胞色素P450(CYP)1A1-MspⅠ、谷胱甘肽硫转移酶(GST)T1基因多态性与口腔癌发病
之间的关系。方法采用病例-对照研究的方法,以300例口腔癌患者(病例组)及300例非癌对照者(对照组)的外周血白细胞为样本,利用聚合酶链反应(PCR)技术检测Ⅰ相代谢酶CYP1A1-MspⅠ和Ⅱ相代谢酶GSTT1基因多态性,并分析吸烟和2种代谢酶基因多态性与口腔癌的相关性。结果病例组CYP1A1-MspⅠ突变纯合型(m2/m2)和GSTT1基因缺陷型[GSTT1(-)]的频率分布分别为38.33%、69.33%,而对照组的频率分布分别为21.00%、44.33%,二者差异有统计学意义(P<0.01)。CYP1A1-MspⅠ(m2/m2)者患口腔癌的风险显著增加(OR=2.34,95%CI为1.76~4.07)。GSTT1(-)者患口腔癌的风险也显著增加(OR=2.84,95%CI为1.98~4.54)。基因突变的协同分析发现CYP1A1-MspⅠ(m2/m2)/GSTT1(-)在病例组和对照组中的分布频率分别为30.67%和6.67%,二者差异有统计学意义(P<0.01)。CYP1A1-MspⅠ(m2/m2)/GSTT1(-)者患口腔癌的风险显著增加(OR=8.27,95%CI为3.63~11.29)。病例组的吸烟率显著高于对照组的吸烟率(OR=2.71,95%CI为1.31~4.52,P<0.01),CYP1A1-MspⅠ(m2/m2)及GSTT1(-)与吸烟有协同作用(OR=25.00,95%CI为11.87~35.64)。结论CYP1A1-MspⅠ(m2/m2)和GSTT1(-)是口腔癌的易感因素,吸烟与口腔癌的易感性也有关,CYP1A1-MspⅠ(m2/m2)和GSTT1(-)与吸烟在口腔癌的发生上有相互促进的作用。

关键词: 口腔癌, 细胞色素P450 1A1-MspⅠ, 谷胱甘肽硫转移酶T1, 多态现象, 吸烟

Abstract:

Objective To investigate the correlation between the combination of smoking and the polymorphisms of cytochrome P450(CYP) 1A1-MspⅠ and glutathione S-transferase(GST) T1 genes and oral cancer. Methods The genetic polymorphisms of CYP1A1-MspⅠ and GSTT1 were detected by polymerase chain reaction(PCR) technique in peripheral blood leukocytes of 300 oral cancer cases and 300 non-cancer controls, and the correlation between smoking, the two metabolic enzymes genetic polymorphisms and oral cancer were analyzed. Results The frequencies of CYP1A1-MspⅠ(m2/m2) and GSTT1(-) were 38.33% and 69.33% in oral cancer cases and 21.00% and 44.33% in healthy controls respectively. Statistical tests showed significant difference in the frequencies between the two groups (P<0.01). The risk of oral cancer with CYP1A1-MspⅠ(m2/m2) was significantly higher than that of controls(OR= 2.34, 95%CI 1.76-4.07). The individuals who carried with GSTT1(-) had a high risk of oral cancer(OR=2.84, 95% CI 1.98-4.54). Combined analysis of the polymorphisms showed that percentage of CYP1A1-MspⅠ(m2/m2)/GSTT1(-)in oral cancer and control groups was 30.67% and 6.67% respectively(P<0.01). The people who carried with CYP1A1-MspⅠ(m2/m2)/GSTT1(-) had a high risk of oral cancer(OR=8.27, 95%CI 3.63-11.29). The smoking rate of the case group was significantly higher than that in the control group(OR=2.71, 95%CI 1.31-4.52, P<0.01), and statistic analysis suggested an interaction between smoking and CYP1A1-MspⅠ(m2/m2)/GSTT1(-) genotypes polymorphisms which increased risk of oral cancer(OR=25.00, 95%CI 11.87- 35.64). Conclusion CYP1A1-MspⅠ(m2/m2) and GSTT1(-)are the risk factors in oral cancer. Smoking is also related to the susceptibility to oral cancer. There may be a synergetic interaction among CYP1A1-MspⅠ(m2/m2), GSTT1(-) and smoking on the elevated susceptibility of oral cancer.

Key words: oral cancer, cytochrome P450 1A1-MspⅠ, glutathione S-transferase T1, polymorphism, smoking