关键词: 咬合创伤, 骨吸收, 基因芯片, 成骨细胞, 破骨细胞

Abstract:

Objective To study the gene expression profiles of traumatic occlusion in early stage with the animal model of rats. Methods The occlusal surface of the upper left first molar of rat was raised by placing a stainless steel wire to induce occlusal trauma in the lower left first molar. After 24 hours, the alveolar bone tissue of the first molars at the both sides of rats’lower jaws were taken out under anesthesia. The different expressive genes were shown by genome-wide microarray, which comprises about 27 000 genes and analyzed the different expressive genes with Pathway and GO analysis, finally the results of the microarray were examined by real time-polymerase chain reaction（RTPCR）. Results In the results of the study, 586 different expressions were found, of which the expressions of 166 genes increased and 420 genes decreased. 106 different pathways were involved with Pathway analysis and 270 different functional classification related to GO analysis. Conclusion The balance of the lower alveolar bone is destroyed after 24 hours of traumatic occlusion. At early phase of the occlusal trauma, osteogenesis and bone formation in alveolar bone are inhibited, yet osteoblast genesis and bone resorption are not significant.

Key words: traumatic occlusion, bone resorption, genome-wide microarray, osteoblast, osteoclast