华西口腔医学杂志

• 基础研究 • 上一篇    下一篇

破骨细胞核因子κB受体活化因子配基和骨保护因子在种植体周围软组织及骨组织的表达

周文娟1 柳忠豪1 许胜1 郝鹏杰1 许丰伟2 孙爱杰1 卢志山1   

  1. 1.烟台市口腔医院种植中心; 2.滨州医学院口腔学院, 烟台264008
  • 收稿日期:2012-02-25 修回日期:2012-02-25 出版日期:2012-02-01 发布日期:2012-02-01
  • 通讯作者: 柳忠豪,Tel:0535-6215913
  • 作者简介:周文娟(1984—),女,山东人,住院医师,硕士
  • 基金资助:

    山东省自然科学基金资助项目(Y2008C43)

Expression of receptor activator nuclear factor kappa B ligand and osteoprotegerin in peri-implant soft tissue and bone tissue

Zhou Wenjuan1, Liu Zhonghao1, Xu Sheng1, Hao Pengjie1, Xu Fengwei2, Sun Aijie1, Lu Zhishan1   

  1. 1. Dental Implantation Centre, Stomatological Hospital of Yantai, Yantai 264008, China; 2. College of Stomatology, Binzhou Medical University, Yantai 264008, China
  • Received:2012-02-25 Revised:2012-02-25 Online:2012-02-01 Published:2012-02-01
  • Contact: Liu Zhonghao,Tel:0535-6215913
  • About author:Zhou Wenjuan(1984—),女,山东人,住院医师,硕士

摘要:

目的研究破骨细胞核因子κB受体活化因子配基(RANKL)及其伪受体骨保护因子(OPG)在非负荷期种植体周围软组织和骨组织中的表达变化及时间分布特点。方法选用6只1~2岁龄的雄性Beagle犬建立种植义齿动物模型,分别观测种植体植入后3、7、15、30、60、90 d种植体周围的骨改建情况。取种植体周围软组织进行实时荧光定量聚合酶链反应(PCR);取犬下颌骨进行大体标本观察拍摄X线片;取种植体周围骨组织进行免疫组化染色,检测RANKL和OPG随时间的表达变化及分布特点。结果骨改建的最活跃期为种植体植入后的第7天,种植体周围软组织中的RANKL和OPG mRNA及骨组织中的RANKL和OPG均随种植体植入时间的增加而增加,第7天达高峰,而后均逐渐降低。RANKL和OPG在种植体周围软组织及骨组织中的表达变化规律一致。结论OPG和RANKL能在种植体周围软组织中表达,且变化规律与种植体周围骨组织改建过程一致。种植体周围组织可以通过OPG/RANKL系统参与破骨细胞的形成,调节骨质吸收,影响骨组织代谢微环境。

关键词: 牙种植, 核因子κB受体活化因子配基, 骨保护因子, 种植体周

Abstract:

Objective To study mRNA expression of receptor activator nuclear factor kappa B ligand(RANKL) and its decoy receptor, osteoprotegerin(OPG) in peri-implant tissue during unloading period. Methods An animal model of dental implant was established in 6 male Beagle dogs of 1-2 years old. Bone remodeling was tested at 3, 7, 15, 30, 60 and 90 days since the placement of implants. RANKL and OPG mRNA expression were quantified by real-time polymerase chain reaction(PCR). Then mandibular bones were taken out and the morphological changes were observed by X-ray, bone tissue was tested by immunohistochemistry stain. Results The most prominent period of bone remodeling occurred at 7th day after the placement of implants. The expression of RANKL and OPG increased in a timedependent manner in both soft and hard tissue. After 7 days they gradually decreased. Conclusion RANKL and OPG can express in soft tissue, and the changing tendency is consistent with the change of bone remodeling, it indicates that RANKL and OPG play an important role in the bone remodeling.

Key words: dental implant, receptor activator nuclear factor kappa B ligand, osteoprotegerin, peri-implant