关键词: 口腔扁平苔藓, 少突胶质细胞系基因-髓鞘碱性蛋白, 外周血单个核细胞

Abstract:

Objective The aim of this study was to determine the expression of genes of the oligodendrocyte lineagemyelin basic protein（Golli-MBP） in peripheral blood mononuclear cell（PBMC） in oral lichen planus（OLP） and to further understand the pathogenesis of OLP. Methods PBMC was obtained by density gradient centrifugation, and the expression of Golli-MBP in PBMC was investigated in erythematous/erosive OLP（20 cases）, reticular OLP（16 cases） and normal controls（19 cases） using reverse transcription-polymerase chain reaction（RT-PCR） and Western blot methods. Results RT-PCR results showed that Golli-MBP mRNA was overexpressed in erythematous/erosive and reticular OLP as compared with normal control group（P<0.01）. Western blot assay indicated that erythematous/erosive and reticular OLP patients had a higher expression level of Golli-MBP protein in PBMC than normal controls（P<0.01）. However, there were no significant differences between erythematous/erosive and reticular groups in Golli-MBP mRNA and protein expression（P>0.05）. Conclusion The data accumulated here strongly indicate that Golli-MBP was involved in the pathogenesis of OLP.

Key words: oral lichen planus, genes of the oligodendrocyte lineage-myelin basic protein, peripheral blood mononuclear cell