华西口腔医学杂志

• 专栏论著 • 上一篇    下一篇

BME-10X型胶原/羟磷灰石人工骨引导比格犬牙周组织再生的实验研究

陈斌1 吴文蕾1 张其清2 黄晓峰3 陈湘华3   

  1. 1.南京大学医学院附属口腔医院牙周科, 南京210008;2.中国医学科学院生物医学工程研究所, 天津市生物医学材料重点实验室, 天津300192;3.南京大学医学院附属口腔医院病理科, 南京210008
  • 收稿日期:2011-10-25 修回日期:2011-10-25 出版日期:2011-10-20 发布日期:2011-10-20
  • 通讯作者: 吴文蕾,Tel:025-83620206
  • 作者简介:陈斌(1984—),女,河南人,住院医师,硕士
  • 基金资助:

    南京市医学科学技术发展专项基金资助项目(重点项目)
    (ZKX07022)

Experimental study on the potential role of BME-10X collagen/hydroxyapatite bone graft in periodontal tissue regeneration of beagle

Chen Bin1, Wu Wenlei1, Zhang Qiqing2, Huang Xiaofeng3, Chen Xianghua3.   

  1. 1. Dept. of Periodontology, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing 210008, China; 2. Institute of Biomedical Engineering, Chinese Academy of Medical Sciences; Tianjin Medical Material Key Laboratory, Tianjin 300192, China; 3. Dept. of Pathology, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing 210008, China
  • Received:2011-10-25 Revised:2011-10-25 Online:2011-10-20 Published:2011-10-20
  • Contact: Wu Wenlei,Tel:025-83620206

摘要:

目的评估BME-10X型胶原/羟磷灰石人工骨引导比格犬牙周组织再生的能力。方法选取18月龄雄性比格犬4只,进行牙周基础治疗。基础治疗结束1周后,选取下颌第3、4前磨牙,同颌对侧同名牙随机纳入实验组或者对照组,制造牙周缺损,实验组植入BME-10X型胶原/羟磷灰石人工骨,对照组不植入任何材料。术后12周处死动物,进行组织病理学分析。结果与对照组相比,实验组获得了较多的组织再生,实验组和对照组新生牙槽骨的高度分别为(3.01±0.14)、(1.32±0.11)mm,二者间差异有统计学意义(P<0.05)。实验组和对照组新生牙周膜组织的高度分别为(3.12±0.19)、(1.35±0.12)mm,二者间差异有统计学意义(P<0.05)。实验组和对照组新生牙骨质的高度分别为(3.30±0.15)、(2.70±0.12)mm,二者间差异无统计学意义(P>0.05)。新生牙周组织的牙周膜纤维深入到新生的骨组织和牙骨质中,其结构特征与生理状态时的结构特征无明显差异。结论BME-10X型胶原/羟磷灰石人工骨材料有较强的诱导牙周组织再生的能力。

关键词: 牙周病, 组织再生, 胶原/羟磷灰石人工骨

Abstract:

Objective To evaluate the potential role of BME-10X collagen/hydroxyapatite(HA) bone graft in periodontal tissue regeneration. Methods Four 18 months old male beagles in the experiment were treated with non-surgical periodontal therapy. At the sites of mandibular 3rd and 4th premolars at the time of dogs were treated with non-surgical periodontal therapy one week later, the teeth with the same name in the same jaw were selected to the experimental group(T group)or the control group(C group) at random. The defects in T group were filled with BME-10X collagen/HA bone graft while the defects in C group were filled with nothing. The dogs were sacrificed in twelve weeks and analyzed by histopathology. Results The defects in T group got more tissue regeneration compared with the defects in C group. The height of new bone(NB)was(3.01±0.14)mm in T group versus(1.32±0.11)mm in C group(P<0.05). The height of new periodontal ligament(NP)was(3.12±0.19)mm in T group versus(1.35±0.12)mm in C group(P<0.05). The height of new cementum(NC) was(3.30±0.15)mm in T group versus(2.70±0.12)mm in C group(P>0.05). The new tissue guided by the bone graft was the same as the normal tissue in histopathology analysis. Conclusion The results of the study suggest that BME-10X collagen/HA bone graft is a good bone graft for periodontal tissue regeneration.

Key words: periodontal disease, tissue regeneration, collagen/hydroxyapatite bone grafts