华西口腔医学杂志

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大鼠舌下神经压榨伤后p38MAPK的活化及外源性神经生长因子促神经再生作用的研究

范丽苑1,2 涂玲1,3   

  1. 1.中南大学湘雅医院口腔科, 湖南长沙410078;2.泸州医学院附属口腔医院修复科, 四川泸州646000;3.中南大学口腔医学院口腔解剖生理教研室, 湖南长沙410078
  • 收稿日期:2010-10-25 修回日期:2010-10-25 出版日期:2010-10-20 发布日期:2010-10-20
  • 通讯作者: 涂玲, Tel:0731-84805319
  • 作者简介:范丽苑(1983—),女,四川人,助教,硕士

The activation of p38MAPK in hypoglossal necleus and the effect of nerve growth factor on regeneration following hypoglossal nerve crush in rat

FAN Li-yuan1,2, TU Ling1,3   

  1. 1. Dept. of Stomatology, Xiangya Hospital, Central South University, Changsha 410078, China; 2. Dept. of Prosthodontics, Stomatological Hospital of Luzhou Medical College, Luzhou 646000, China; 3. Dept. of Anatomy and Physiology, College of Stomatology, Central South University, Changsha 410078, China
  • Received:2010-10-25 Revised:2010-10-25 Online:2010-10-20 Published:2010-10-20
  • Contact: TU Ling, Tel:0731-84805319

摘要:

目的检测舌下神经压榨损伤前后及神经生长因子(NGF)干预后磷酸化p38MAPK的表达,探讨p38MAPK在大鼠舌下神经损伤及干预后的作用和NGF对大鼠舌下神经损伤后神经修复再生的作用。方法60只SD大鼠随机分为正常对照组(NC组)、实验对照组(NS组)和NGF治疗组(NGF组),动物存活时间分别为1、3、5、7和14 d。分别于各时间点取脑干做免疫组化测定及Nissl染色,取神经干做透射电镜观察。结果舌下神经压榨损伤后,舌下神经核内磷酸化p38MAPK免疫阳性神经元数目和染色深度均增加(P<0.05)。NGF干预后舌下神经核内磷酸化p38MAPK免疫阳性神经元数目和染色深度均明显减少(P<0.05)。Nissl染色显示,术后7、14 d NGF组损伤侧舌下神经核内运动神经元存活率明显高于NS组。透射电镜观察NGF组神经干形态优于NS组。结论大鼠舌下神经压榨损伤后受损神经元p38MAPK的活性增强;外源性NGF能抑制大鼠舌下神经压榨损伤引起的舌下神经核运动神经元p38MAPK的激活;大鼠舌下神经压榨损伤后外源性NGF具有保护受损的舌下神经元及促进神经再生的作用。

关键词: 神经生长因子, 舌下神经, 压榨损伤, 再生

Abstract:

Objective To explore the activation of phospho-p38 mitogen activated protein kinases(p-p38MAPK) and to evaluate the possible role of nerve growth factor(NGF) on neuronal protection and regeneration of injured nerve in a model of hypoglossal nerve injury in rats. Methods Sixty healthy adult SD rats were divided randomly into three groups: NC control group, NS control group and NGF treatment group. The rats were allowed to survive for 1, 3, 5, 7 and 14 days after operation respectively. Frozen sections were processed for immunohistochemistry(IHC) to decide the p-p38MAPK expression level in the motoneurons of hypoglossal nucleus. Nissl’s staining was used to evaluate cellular morphological and architectural changes in the hypoglossal nucleus. Transmission electron microscope (TEM) study was employed to investigate the subcellular structural alternations of the hypoglossal nerve distal to the injury site. Results The expression level of p-p38MAPK was low in NC group and elevated in all operated animals. However, p-p38MAPK immunoreactivity in the hypoglossal motoneurons in NGF group was lower than NS control group after injury. The survival rate of motoneurons in hypoglossal nucleus of injured side in NGF group was higher than that in NS group. Ultrastructural study revealed more regenerating myelinated axons which distributed homogenously in the distal site of the lesioned hypoglossal nerve from the NGF group than NS group. Conclusion p-p38MAPK was slightly expression in normal hypoglossal nucleus but intensively expression after injury. NGF candown-regulate p-p38MAPK expression in the motoneurons of hypoglossal nucleus after hypoglossal nerve was crushed. Exogenous NGF can protect damaged neurons and promote nerve regeneration after hypoglossal nerve crush injury in rats.

Key words: nerve growth factor, hypoglossal nerve, crush, regeneration