华西口腔医学杂志

• 基础研究 • 上一篇    下一篇

口腔鳞状细胞癌线粒体DNA高变Ⅱ区及高变Ⅲ区突变的功能意义

王耀钟 贾暮云 袁荣涛 韩国栋 卜令学   

  1. 青岛大学医学院附属医院口腔颌面外科, 山东青岛266003
  • 收稿日期:2010-06-25 修回日期:2010-06-25 出版日期:2010-06-20 发布日期:2010-06-20
  • 通讯作者: 贾暮云,Tel:13589268356
  • 作者简介:王耀钟(1983—),男,山东人,硕士
  • 基金资助:

    上海市科学技术委员会基金资助项目(08DZ2271100);上海市重点学科建设基金资助项目(S30206-Kf13)

The mutations of the D-loop hypervariable region Ⅱ and hypervariable region Ⅲ of mitochondrial DNA in ral squamous cell carcinoma

WANG Yao-zhong, JIA Mu-yun, YUAN Rong-tao, HAN Guo-dong, BU Ling-xue   

  1. Dept. of Oral and Maxillofacial Surgery, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China
  • Received:2010-06-25 Revised:2010-06-25 Online:2010-06-20 Published:2010-06-20
  • Contact: JIA Mu-yun,Tel:13589268356

摘要:

目的检测口腔鳞状细胞癌线粒体DNA(mtDNA)复制控制区D环(D-loop)区的高变Ⅱ区(HVR Ⅱ)及高变Ⅲ区(HVR Ⅲ)的突变情况,并探讨其意义。方法以癌旁组织和正常组织为对照,对7例口腔鳞状细胞癌组织样本的mtDNA D-loop HVR Ⅱ区及HVR Ⅲ区进行聚合酶链反应(PCR)扩增和测序分析。结果在7例患者的癌组织、旁组织、正常组织样本中共发现82个(56种)核苷酸改变,其中51个(26种)为核苷酸多态性改变;3个肿瘤组织样中共发现31个(30种)突变,其中21个位于HVR Ⅱ区及HVR Ⅲ区范围内;癌旁组织及正常组织未发现突变;口 鳞状细胞癌的mtDNA D-loop HVR Ⅱ区及HVR Ⅲ区突变率为42.9%(3/7)。结论mtDNA D-loop HVR Ⅱ区及HVR Ⅲ区的突变与口腔鳞状细胞癌的发生相关,为寻找新的肿瘤基因诊断和肿瘤遗传易感性的标志物提供了依据。

关键词: 鳞状细胞癌, 线粒体DNA, 突变

Abstract:

Objective To investigate the frequency of mitochondrial DNA(mtDNA) D-loop hypervariable region Ⅱ  HVR Ⅱ) and hypervariable region Ⅲ(HVR Ⅲ) mutations in oral squamous cell carcinoma(OSCC) and their orrelation to provide the new targets for the prevention and treatment of OSCC. Methods The D-loop HVR Ⅱ and VR Ⅲ regions of mtDNA in seven cases with OSCC tissues, matched with paracancerous tissues and normal mucosa issues from the same case, were amplified by polymerase chain raction(PCR), then were detected by direct equencing to find the mutantsites after the comparison of all sequencing results with the mtDNA Cambridge sequence n the GenBank database. Results 82(56 species) nucleotide changes, with 51(26 species) nucleotide polymor - hism, were found after the comparison of all sequencing results with the mtDNA Cambridge sequence in the Gen- ank database. 31(30 species) mutations, with 21 located within the HVR Ⅱ and HVR Ⅲ regions, were found in 3 umor tissue samples, their paracancerous and normal mucosa tissue were found more polymorphic changes but no utation. The mtDNA D-loop HVR Ⅱ and HVR Ⅲ regions mutation rate was 42.9%(3/7) in OSCC. Conclusion The tDNA D-loop HVR Ⅱ and HVR Ⅲ regions were highly polymorphic and mutable regions in OSCC. It suggested hat the D -loop HVR Ⅱ and HVR Ⅲ regions of mtDNA might play a significant role in the tumorigenesis f OSCC. It may become new targets for the gene therapy of OSCC by regulating the above indexes.

Key words: squamous cell carcinoma, mitochondrial DNA, mutation