华西口腔医学杂志

• 基础研究 • 上一篇    下一篇

5种牙体修复材料对L929细胞凋亡及相关基因的影响

王学1 章非敏1,2 刘梅1 殷雪萌1 顾宁2 光寒冰3   

  1. 1.南京医科大学口腔医学研究所, 江苏南京210029;2.东南大学苏州研究院, 江苏苏州215123; 3.江苏省口腔医院义齿加工中心, 江苏南京210029
  • 收稿日期:2010-06-25 修回日期:2010-06-25 出版日期:2010-06-20 发布日期:2010-06-20
  • 通讯作者: 章非敏,Tel:025-85031831
  • 作者简介:王学(1983—),女,江苏人,硕士
  • 基金资助:

    江苏省国际科技合作计划基金资助项目(BZ2008059);江苏省自然科学基金资助项目(BK2009137)

Induction of apoptosis and related genes by five kinds of dental materials on L929

WANG Xue1,ZHANG Fei-min1,2, LIU Mei1, YIN Xue-meng1, GU Ning2, GUANG Han-bing3   

  1. 1. Institute of Stomatology, Nanjing edical University, Nanjing 210029, China; 2. Suzhou Institute, Southeast University, Suzhou 215123, China; 3. enture Machining Center, Stomatology Hospital of Jiangsu Province, Nanjing 210029, China
  • Received:2010-06-25 Revised:2010-06-25 Online:2010-06-20 Published:2010-06-20
  • Contact: ZHANG Fei-min,Tel:025-85031831

摘要:

目的研究新型硅藻土可切削陶瓷及临床常用口腔修复材料对L929细胞致凋亡作用。方法提取新型硅藻土可切削陶瓷及其他4种口腔材料的浸提液作用于细胞,流式细胞仪检测细胞周期进程及诱导细胞凋亡作用;Annexin V-FITC/PI双染细胞凋亡检测试剂盒对细胞死亡类型进行检测;逆转录聚合酶链反应检测Bcl-2和Bax基因的表达。结果各实验组对细胞周期均无特殊影响,新型陶瓷组凋亡率与阴性组接近(P>0.05)。树脂组凋亡率最高,细胞坏死水平也显著提高,与新型陶瓷组间差异有统计学意义(P<0.05)。新型陶瓷组Bcl-2和Bax mRNA表达水平与阴性组间差异无统计学意义(P>0.05)。结论新型硅藻土可切削陶瓷无明显细胞毒性,符合临床应用要求。

关键词: 硅藻土可切削陶瓷, L929细胞, 细胞凋亡

Abstract:

Objective To evaluate a new type of diatomite-based machinable ceramic biocompatibility by studying its induced apoptosis on L929 cell in contrasted with other prosthodontics materials. Methods Cell line was treated with extracting liquid containing different concentrations of diatomite-based machinable ceramic and other materials. Flow cytometry tested cell cycle progression and induced cell apoptosis. Annexin V-FITC/PI apoptosis staining kit quantitative detected cell death patterns. The expression of Bcl -2 and Bax mRNA were determined by reverse transcription -polymerase chain raction. Results The experimental groups had no special influence on cell cycle. Apoptosis rates of the new ceramic closed to the negative group(P>0.05). The apoptosis rate of resin was the highest, and the cell necrosis level of resin was increased, which had significant difference to the new ceramic(P<0.05). The Bcl-2 and Bax mRNA levels of the new ceramic and the negative group were closed to each other, which had no statistical significance(P>0.05). Conclusion The new diatomite-based machinable ceramic has no apparent cytotoxicity, which is consistent with the clinical application of the basic requirements of biocompatibility.

Key words: diatomite-based machinable ceramic, L929 cell, apoptosis